Tags

Type your tag names separated by a space and hit enter

Purification of wheat glutenin subunits by preparative acid and two-dimensional electrophoresis.
Electrophoresis. 1995 Jun; 16(6):1005-9.E

Abstract

Reduced alkylated glutenin subunits from wheat flour were fractionated by preparative electrophoresis at acid pH. The high molecular weight glutenin subunits (HMW-GS) and some of the low molecular weight glutenin subunits (LMW-GS) were purified by this one-step procedure, whereas the remainder of the LMW-GS, comigrating in the acid system, were purified in a second step by electroendosmotic preparative electrophoresis in the presence of sodium dodecyl sulfate. The quantities of recovered protein were sufficient for biochemical characterization and/or antibody production.

Links

Publisher Full Text

Authors+Show Affiliations

Curioni A
Dipartimento di Biotecnologie Agrarie, Università di Padova, Italy.
Morel MH
No affiliation info available
Furegon L
No affiliation info available
Redaelli R
No affiliation info available
Dal Belin Peruffo A
No affiliation info available

MeSH

AcetatesAcetic AcidAlkylationElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelGlutensHydrogen-Ion ConcentrationTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7498120

Citation

Curioni, A, et al. "Purification of Wheat Glutenin Subunits By Preparative Acid and Two-dimensional Electrophoresis." Electrophoresis, vol. 16, no. 6, 1995, pp. 1005-9.
Curioni A, Morel MH, Furegon L, et al. Purification of wheat glutenin subunits by preparative acid and two-dimensional electrophoresis. Electrophoresis. 1995;16(6):1005-9.
Curioni, A., Morel, M. H., Furegon, L., Redaelli, R., & Dal Belin Peruffo, A. (1995). Purification of wheat glutenin subunits by preparative acid and two-dimensional electrophoresis. Electrophoresis, 16(6), 1005-9.
Curioni A, et al. Purification of Wheat Glutenin Subunits By Preparative Acid and Two-dimensional Electrophoresis. Electrophoresis. 1995;16(6):1005-9. PubMed PMID: 7498120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of wheat glutenin subunits by preparative acid and two-dimensional electrophoresis. AU - Curioni,A, AU - Morel,M H, AU - Furegon,L, AU - Redaelli,R, AU - Dal Belin Peruffo,A, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 1005 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 16 IS - 6 N2 - Reduced alkylated glutenin subunits from wheat flour were fractionated by preparative electrophoresis at acid pH. The high molecular weight glutenin subunits (HMW-GS) and some of the low molecular weight glutenin subunits (LMW-GS) were purified by this one-step procedure, whereas the remainder of the LMW-GS, comigrating in the acid system, were purified in a second step by electroendosmotic preparative electrophoresis in the presence of sodium dodecyl sulfate. The quantities of recovered protein were sufficient for biochemical characterization and/or antibody production. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/7498120/Purification_of_wheat_glutenin_subunits_by_preparative_acid_and_two_dimensional_electrophoresis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0173-0835&date=1995&volume=16&issue=6&spage=1005 DB - PRIME DP - Unbound Medicine ER -