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The role of DNA repair genes in recombination between repeated sequences in yeast.
Genetics. 1995 Aug; 140(4):1199-211.G

Abstract

The presence of repeated sequences in the genome represents a potential source of karyotypic instability. Genetic control of recombination is thus important to preserve the integrity of the genome. To investigate the genetic control of recombination between repeated sequences, we have created a series of isogenic strains in which we could assess the role of genes involved in DNA repair in two types of recombination: direct repeat recombination and ectopic gene conversion. Naturally occurring (Ty elements) and artificially constructed repeats could be compared in the same cell population. We have found that direct repeat recombination and gene conversion have different genetic requirements. The role of the RAD51, RAD52, RAD54, RAD55, and RAD57 genes, which are involved in recombinational repair, was investigated. Based on the phenotypes of single and double mutants, these genes can be divided into three functional subgroups: one composed of RAD52, a second one composed of RAD51 and RAD54, and a third one that includes the RAD55 and RAD57 genes. Among seven genes involved in excision repair tested, only RAD1 and RAD10 played a role in the types of recombination studied. We did not detect a differential effect of any rad mutation on Ty elements as compared to artificially constructed repeats.

Authors+Show Affiliations

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7498763

Citation

Liefshitz, B, et al. "The Role of DNA Repair Genes in Recombination Between Repeated Sequences in Yeast." Genetics, vol. 140, no. 4, 1995, pp. 1199-211.
Liefshitz B, Parket A, Maya R, et al. The role of DNA repair genes in recombination between repeated sequences in yeast. Genetics. 1995;140(4):1199-211.
Liefshitz, B., Parket, A., Maya, R., & Kupiec, M. (1995). The role of DNA repair genes in recombination between repeated sequences in yeast. Genetics, 140(4), 1199-211.
Liefshitz B, et al. The Role of DNA Repair Genes in Recombination Between Repeated Sequences in Yeast. Genetics. 1995;140(4):1199-211. PubMed PMID: 7498763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of DNA repair genes in recombination between repeated sequences in yeast. AU - Liefshitz,B, AU - Parket,A, AU - Maya,R, AU - Kupiec,M, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 1199 EP - 211 JF - Genetics JO - Genetics VL - 140 IS - 4 N2 - The presence of repeated sequences in the genome represents a potential source of karyotypic instability. Genetic control of recombination is thus important to preserve the integrity of the genome. To investigate the genetic control of recombination between repeated sequences, we have created a series of isogenic strains in which we could assess the role of genes involved in DNA repair in two types of recombination: direct repeat recombination and ectopic gene conversion. Naturally occurring (Ty elements) and artificially constructed repeats could be compared in the same cell population. We have found that direct repeat recombination and gene conversion have different genetic requirements. The role of the RAD51, RAD52, RAD54, RAD55, and RAD57 genes, which are involved in recombinational repair, was investigated. Based on the phenotypes of single and double mutants, these genes can be divided into three functional subgroups: one composed of RAD52, a second one composed of RAD51 and RAD54, and a third one that includes the RAD55 and RAD57 genes. Among seven genes involved in excision repair tested, only RAD1 and RAD10 played a role in the types of recombination studied. We did not detect a differential effect of any rad mutation on Ty elements as compared to artificially constructed repeats. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/7498763/The_role_of_DNA_repair_genes_in_recombination_between_repeated_sequences_in_yeast_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/7498763/ DB - PRIME DP - Unbound Medicine ER -