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Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain.
Arch Pathol Lab Med. 1993 Dec; 117(12):1249-51.AP

Abstract

Light microscopic diagnosis of intestinal microsporidiosis is difficult with the use of routine histologic stains. This has led to an overreliance on transmission electron microscopic diagnosis. It was previously demonstrated that a modification of the standard Gomori one-step trichrome stain, using a 10-fold higher concentration of chromotrope-2R, can be used to detect microsporidial spores in stool. The use of the stain has now been extended to the detection of spores in sections of formaldehyde-fixed, paraffin-embedded intestinal biopsy specimens. Positive identification can be made of both intestinal species seen in patients with the acquired immunodeficiency syndrome, Enterocytozoon bieneusi and Septata intestinalis, when the diagnosis is inapparent or questionable on routine histologic analysis. The use of this simple stain should increase the sensitivity for diagnosing microsporidiosis by light microscopy, further obviating the need for transmission electron microscopy.

Authors+Show Affiliations

Department of Medicine, St Luke's/Roosevelt Hospital Center, College of Physicians and Surgeons, Columbia University, New York, NY 10025.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

7504441

Citation

Giang, T T., et al. "Tissue Diagnosis of Intestinal Microsporidiosis Using the chromotrope-2R Modified Trichrome Stain." Archives of Pathology & Laboratory Medicine, vol. 117, no. 12, 1993, pp. 1249-51.
Giang TT, Kotler DP, Garro ML, et al. Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain. Arch Pathol Lab Med. 1993;117(12):1249-51.
Giang, T. T., Kotler, D. P., Garro, M. L., & Orenstein, J. M. (1993). Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain. Archives of Pathology & Laboratory Medicine, 117(12), 1249-51.
Giang TT, et al. Tissue Diagnosis of Intestinal Microsporidiosis Using the chromotrope-2R Modified Trichrome Stain. Arch Pathol Lab Med. 1993;117(12):1249-51. PubMed PMID: 7504441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain. AU - Giang,T T, AU - Kotler,D P, AU - Garro,M L, AU - Orenstein,J M, PY - 1993/12/1/pubmed PY - 1993/12/1/medline PY - 1993/12/1/entrez SP - 1249 EP - 51 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 117 IS - 12 N2 - Light microscopic diagnosis of intestinal microsporidiosis is difficult with the use of routine histologic stains. This has led to an overreliance on transmission electron microscopic diagnosis. It was previously demonstrated that a modification of the standard Gomori one-step trichrome stain, using a 10-fold higher concentration of chromotrope-2R, can be used to detect microsporidial spores in stool. The use of the stain has now been extended to the detection of spores in sections of formaldehyde-fixed, paraffin-embedded intestinal biopsy specimens. Positive identification can be made of both intestinal species seen in patients with the acquired immunodeficiency syndrome, Enterocytozoon bieneusi and Septata intestinalis, when the diagnosis is inapparent or questionable on routine histologic analysis. The use of this simple stain should increase the sensitivity for diagnosing microsporidiosis by light microscopy, further obviating the need for transmission electron microscopy. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/7504441/Tissue_diagnosis_of_intestinal_microsporidiosis_using_the_chromotrope_2R_modified_trichrome_stain_ L2 - http://www.diseaseinfosearch.org/result/4799 DB - PRIME DP - Unbound Medicine ER -