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Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells.
Clin Exp Immunol. 1977 Oct; 30(1):160-7.CE

Abstract

A crude plasma membrane fraction from the homogenate of purified rat mast cells demonstrates a high degree of Ca2+-dependent and Mg2+-dependent adenosine triphosphatase (ATPase) activity. The microsomal and mitochondrial fractions show negligible amounts of the Ca2+ and Mg2+-activated ATPases. The broad ATPase inhibitor, ethacrynic acid, effectively blocks the mast cell ATPase activity while ouabain demonstrates little inhibitory effect. Correspondingly, ethacrynic acid inhibits histamine release from antigen-challenged mast cells while ouabain does not. Both ATPase inhibition and histamine release inhibition by ethacrynic acid require the presence of the olefinic bond in the ethacrynic acid molecule.

Authors

No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

75076

Citation

Magro, A M.. "Ethacrynic Acid Inhibitable Ca2+ and Mg2+-activated Membrane Adenosine Triphosphatase in Rat Mast Cells." Clinical and Experimental Immunology, vol. 30, no. 1, 1977, pp. 160-7.
Magro AM. Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells. Clin Exp Immunol. 1977;30(1):160-7.
Magro, A. M. (1977). Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells. Clinical and Experimental Immunology, 30(1), 160-7.
Magro AM. Ethacrynic Acid Inhibitable Ca2+ and Mg2+-activated Membrane Adenosine Triphosphatase in Rat Mast Cells. Clin Exp Immunol. 1977;30(1):160-7. PubMed PMID: 75076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells. A1 - Magro,A M, PY - 1977/10/1/pubmed PY - 1977/10/1/medline PY - 1977/10/1/entrez SP - 160 EP - 7 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 30 IS - 1 N2 - A crude plasma membrane fraction from the homogenate of purified rat mast cells demonstrates a high degree of Ca2+-dependent and Mg2+-dependent adenosine triphosphatase (ATPase) activity. The microsomal and mitochondrial fractions show negligible amounts of the Ca2+ and Mg2+-activated ATPases. The broad ATPase inhibitor, ethacrynic acid, effectively blocks the mast cell ATPase activity while ouabain demonstrates little inhibitory effect. Correspondingly, ethacrynic acid inhibits histamine release from antigen-challenged mast cells while ouabain does not. Both ATPase inhibition and histamine release inhibition by ethacrynic acid require the presence of the olefinic bond in the ethacrynic acid molecule. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/75076/Ethacrynic_acid_inhibitable_Ca2+_and_Mg2+_activated_membrane_adenosine_triphosphatase_in_rat_mast_cells_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/75076/ DB - PRIME DP - Unbound Medicine ER -