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Genetic and molecular characterization of the Escherichia coli secD operon and its products.
J Bacteriol 1994; 176(3):804-14JB

Abstract

The secD operon of Escherichia coli is required for the efficient export of proteins. We have characterized this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports. An analysis of yajC mutations constructed in vitro and recombined onto the chromosome indicates that yajC is neither essential nor a sec gene. The secD operon is not induced in response to either secretion defects or temperature changes. TnphoA fusions have been used to analyze the topology of SecD in the inner membrane; the protein contains six transmembrane stretches and a large periplasmic domain. TnphoA fusions to SecD and SecF have also been recombined onto the chromosome and used to determine the level of these proteins within the cell. Our results indicate that there are fewer than 30 SecD and SecF molecules per cell.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7507921

Citation

Pogliano, K J., and J Beckwith. "Genetic and Molecular Characterization of the Escherichia Coli secD Operon and Its Products." Journal of Bacteriology, vol. 176, no. 3, 1994, pp. 804-14.
Pogliano KJ, Beckwith J. Genetic and molecular characterization of the Escherichia coli secD operon and its products. J Bacteriol. 1994;176(3):804-14.
Pogliano, K. J., & Beckwith, J. (1994). Genetic and molecular characterization of the Escherichia coli secD operon and its products. Journal of Bacteriology, 176(3), pp. 804-14.
Pogliano KJ, Beckwith J. Genetic and Molecular Characterization of the Escherichia Coli secD Operon and Its Products. J Bacteriol. 1994;176(3):804-14. PubMed PMID: 7507921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and molecular characterization of the Escherichia coli secD operon and its products. AU - Pogliano,K J, AU - Beckwith,J, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 804 EP - 14 JF - Journal of bacteriology JO - J. Bacteriol. VL - 176 IS - 3 N2 - The secD operon of Escherichia coli is required for the efficient export of proteins. We have characterized this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports. An analysis of yajC mutations constructed in vitro and recombined onto the chromosome indicates that yajC is neither essential nor a sec gene. The secD operon is not induced in response to either secretion defects or temperature changes. TnphoA fusions have been used to analyze the topology of SecD in the inner membrane; the protein contains six transmembrane stretches and a large periplasmic domain. TnphoA fusions to SecD and SecF have also been recombined onto the chromosome and used to determine the level of these proteins within the cell. Our results indicate that there are fewer than 30 SecD and SecF molecules per cell. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/7507921/Genetic_and_molecular_characterization_of_the_Escherichia_coli_secD_operon_and_its_products_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=7507921 DB - PRIME DP - Unbound Medicine ER -