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Transcription by T7 RNA polymerase of DNA containing abasic sites.
Environ Mol Mutagen 1994; 23(1):32-6EM

Abstract

The effects of abasic (AP) sites on RNA synthesis were studied in vitro, using T7 RNA polymerase and a plasmid template containing a T7 promoter. The presence of increasing numbers of AP sites caused a progressive decline in RNA synthesis. The average RNA chain length, calculated from the ratio of initiation to chain elongation, decreased with increasing numbers of AP sites, revealing that complete blocks must occur during synthesis. The probability that RNA polymerase would be blocked at an AP site in the DNA template strand was estimated to be 0.3 in our experimental conditions. These results demonstrate that RNA synthesis by T7 RNA polymerase is inhibited by AP sites and that readthrough of the lesion occurs more frequently than premature chain termination. Chemical reduction of AP sites in the template did not change the block/bypass pattern.

Authors+Show Affiliations

Département de Biochimie, Université de Montréal, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7510226

Citation

Sanchez, G, and M D. Mamet-Bratley. "Transcription By T7 RNA Polymerase of DNA Containing Abasic Sites." Environmental and Molecular Mutagenesis, vol. 23, no. 1, 1994, pp. 32-6.
Sanchez G, Mamet-Bratley MD. Transcription by T7 RNA polymerase of DNA containing abasic sites. Environ Mol Mutagen. 1994;23(1):32-6.
Sanchez, G., & Mamet-Bratley, M. D. (1994). Transcription by T7 RNA polymerase of DNA containing abasic sites. Environmental and Molecular Mutagenesis, 23(1), pp. 32-6.
Sanchez G, Mamet-Bratley MD. Transcription By T7 RNA Polymerase of DNA Containing Abasic Sites. Environ Mol Mutagen. 1994;23(1):32-6. PubMed PMID: 7510226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcription by T7 RNA polymerase of DNA containing abasic sites. AU - Sanchez,G, AU - Mamet-Bratley,M D, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 32 EP - 6 JF - Environmental and molecular mutagenesis JO - Environ. Mol. Mutagen. VL - 23 IS - 1 N2 - The effects of abasic (AP) sites on RNA synthesis were studied in vitro, using T7 RNA polymerase and a plasmid template containing a T7 promoter. The presence of increasing numbers of AP sites caused a progressive decline in RNA synthesis. The average RNA chain length, calculated from the ratio of initiation to chain elongation, decreased with increasing numbers of AP sites, revealing that complete blocks must occur during synthesis. The probability that RNA polymerase would be blocked at an AP site in the DNA template strand was estimated to be 0.3 in our experimental conditions. These results demonstrate that RNA synthesis by T7 RNA polymerase is inhibited by AP sites and that readthrough of the lesion occurs more frequently than premature chain termination. Chemical reduction of AP sites in the template did not change the block/bypass pattern. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/7510226/Transcription_by_T7_RNA_polymerase_of_DNA_containing_abasic_sites_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0893-6692&date=1994&volume=23&issue=1&spage=32 DB - PRIME DP - Unbound Medicine ER -