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Structure and expression of Spk-1, an src-related gene product found in the planarian Dugesia (G) tigrina.

Abstract

A cDNA clone encoding a 497 amino acid protein 75% similar to most Src proteins has been isolated from the planarian Dugesia (Girardia) tigrina (Platyhelminthes; Turbellaria) by PCR followed by screening procedures. This gene product has been designated Spk-1 as it is the first Src-related kinase isolated in a planarian. The predicted amino acid sequence of Spk-1 suggest that it is anchored to the plasma membrane and that it interacts with other phosphotyrosine proteins. Spk-1 is expressed in both intact and regenerating organisms as an mRNA transcript of about 1.9 kb. Planarians, which conserve most features of the common ancestor to protostomian and deuterostomian phyla, are the most primitive triploblastic organisms from which a protein tyrosine kinase gene product has been isolated. The presence of this gene product in such a primitive organism, and its presumed role, are discussed.

Links

Authors+Show Affiliations

,

Department de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.

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Source

Oncogene 9:4 1994 Apr pg 1267-72

MeSH

Amino Acid Sequence
Animals
Base Sequence
Biological Evolution
Blotting, Northern
Genes, src
Molecular Sequence Data
Planarians
Polymerase Chain Reaction
Protein-Tyrosine Kinases
RNA
Regeneration
src-Family Kinases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7510865

Citation

TY - JOUR T1 - Structure and expression of Spk-1, an src-related gene product found in the planarian Dugesia (G) tigrina. AU - Burgaya,F, AU - Garcia-Fernàndez,J, AU - Riutort,M, AU - Baguñà,J, AU - Saló,E, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez SP - 1267 EP - 72 JF - Oncogene JO - Oncogene VL - 9 IS - 4 N2 - A cDNA clone encoding a 497 amino acid protein 75% similar to most Src proteins has been isolated from the planarian Dugesia (Girardia) tigrina (Platyhelminthes; Turbellaria) by PCR followed by screening procedures. This gene product has been designated Spk-1 as it is the first Src-related kinase isolated in a planarian. The predicted amino acid sequence of Spk-1 suggest that it is anchored to the plasma membrane and that it interacts with other phosphotyrosine proteins. Spk-1 is expressed in both intact and regenerating organisms as an mRNA transcript of about 1.9 kb. Planarians, which conserve most features of the common ancestor to protostomian and deuterostomian phyla, are the most primitive triploblastic organisms from which a protein tyrosine kinase gene product has been isolated. The presence of this gene product in such a primitive organism, and its presumed role, are discussed. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/7510865/Structure_and_expression_of_Spk_1_an_src_related_gene_product_found_in_the_planarian_Dugesia<strong>G</strong>tigrina_ ER -