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IL-1 alpha and TNF alpha act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains.
J Cell Physiol. 1994 May; 159(2):221-8.JC

Abstract

Human bone marrow stromal cells respond to stimulation by the monokines IL-1 and TNF by producing colony-stimulating factors such as GM-CSF and G-CSF. In this study we show that IL-1 alpha and TNF alpha act synergistically to stimulate GM-CSF and G-CSF production by cultured marrow stromal cells. We further show that IL-1 alpha and TNF alpha synergistically stimulate production of GM-CSF and G-CSF by a clonal stroma-derived cell strain. Although IL-1 and TNF share many of the same biological activities, we show that IL-1 alpha and TNF alpha have an unequal ability to induce myeloid-CSF production by both cultures, with IL-1 alpha being the more potent inducer. We found that induction by IL-1 alpha and TNF alpha was independent of cell proliferation. The effect of IL-1 alpha and TNF alpha on production of the two myeloid-CSFs by the clonal cells was significantly greater than the unfractionated passaged stromal cultures, having the greater effect on G-CSF production. The clonally derived stromal cells constitutively produced colony-stimulating activity, in particular GM-CSF, at levels easily detected by ELISA. These findings show that, in addition to the overlapping and additive activities of IL-1 alpha and TNF alpha, they can interact synergistically. Our findings further suggest that a small subpopulation of stroma cells may be the major producer of G-CSF in the marrow microenvironment during immune response.

Authors+Show Affiliations

Department of Internal Medicine and Pediatrics, University of Michigan, Ann Arbor 48109.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7512974

Citation

Caldwell, J, and S G. Emerson. "IL-1 Alpha and TNF Alpha Act Synergistically to Stimulate Production of Myeloid Colony-stimulating Factors By Cultured Human Bone Marrow Stromal Cells and Cloned Stromal Cell Strains." Journal of Cellular Physiology, vol. 159, no. 2, 1994, pp. 221-8.
Caldwell J, Emerson SG. IL-1 alpha and TNF alpha act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains. J Cell Physiol. 1994;159(2):221-8.
Caldwell, J., & Emerson, S. G. (1994). IL-1 alpha and TNF alpha act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains. Journal of Cellular Physiology, 159(2), 221-8.
Caldwell J, Emerson SG. IL-1 Alpha and TNF Alpha Act Synergistically to Stimulate Production of Myeloid Colony-stimulating Factors By Cultured Human Bone Marrow Stromal Cells and Cloned Stromal Cell Strains. J Cell Physiol. 1994;159(2):221-8. PubMed PMID: 7512974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-1 alpha and TNF alpha act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains. AU - Caldwell,J, AU - Emerson,S G, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 221 EP - 8 JF - Journal of cellular physiology JO - J Cell Physiol VL - 159 IS - 2 N2 - Human bone marrow stromal cells respond to stimulation by the monokines IL-1 and TNF by producing colony-stimulating factors such as GM-CSF and G-CSF. In this study we show that IL-1 alpha and TNF alpha act synergistically to stimulate GM-CSF and G-CSF production by cultured marrow stromal cells. We further show that IL-1 alpha and TNF alpha synergistically stimulate production of GM-CSF and G-CSF by a clonal stroma-derived cell strain. Although IL-1 and TNF share many of the same biological activities, we show that IL-1 alpha and TNF alpha have an unequal ability to induce myeloid-CSF production by both cultures, with IL-1 alpha being the more potent inducer. We found that induction by IL-1 alpha and TNF alpha was independent of cell proliferation. The effect of IL-1 alpha and TNF alpha on production of the two myeloid-CSFs by the clonal cells was significantly greater than the unfractionated passaged stromal cultures, having the greater effect on G-CSF production. The clonally derived stromal cells constitutively produced colony-stimulating activity, in particular GM-CSF, at levels easily detected by ELISA. These findings show that, in addition to the overlapping and additive activities of IL-1 alpha and TNF alpha, they can interact synergistically. Our findings further suggest that a small subpopulation of stroma cells may be the major producer of G-CSF in the marrow microenvironment during immune response. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/7512974/IL_1_alpha_and_TNF_alpha_act_synergistically_to_stimulate_production_of_myeloid_colony_stimulating_factors_by_cultured_human_bone_marrow_stromal_cells_and_cloned_stromal_cell_strains_ L2 - https://doi.org/10.1002/jcp.1041590205 DB - PRIME DP - Unbound Medicine ER -