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Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules.
APMIS. 1994 Jan; 102(1):23-37.A

Abstract

Serial biopsies from 41 consecutive renal allotransplanted patients were evaluated in order to obtain pretransplant data as well as information on well-functioning and acutely rejecting grafts. Each patient served as his own control. Thirty-five patients were followed according to the schedule which included biopsy prior to transplantation, shortly after opening of reanastomosis, at least once postoperatively (days 7-10), and furthermore whenever clinically indicated. The morphological evaluation was in each case combined with immunofluorescence (to detect immunoglobulins and complement fractions) and immunohistochemistry with a wide panel of monoclonal antibodies for T cells (CD2, CD3, CD4, CD8, gamma delta), B cells (CD20, CD22), macrophages (CD68, MAC387) NK cells (leu-7, CD16), activation markers (IL-2-R, Ki-67, transferrin-R), MHC antigens (HLA-ABC, HLA-DR), adhesion molecules (ICAM-1, VCAM-1, ELAM-1, PADGEM, VLA-4, LFA-1 alpha/beta), and growth factors (EGF, TGF-alpha, EGF-R). When 132 biopsies and 10 failed allografts were examined, no specific morphological or immunohistological parameter predictive of rejection or graft outcome could be found. Morphology in follow-up biopsies from non-rejecting and rejecting patients revealed a continuum of inflammatory changes, and several non-rejecting cases demonstrated cellular inflammatory infiltrates which could not be discriminated from those seen in acute rejection. Of the patients 44% had acute rejection accompanied by increased infiltration of T cells and macrophages showing enhanced IL-2-R expression, increased tubular and endothelial staining for MHC class II, ICAM-1, and VCAM-1, and strong leukocytic expression of VLA-4 and LFA-1 alpha/beta.

Authors+Show Affiliations

Department of Pathology, Herlev Hospital, University of Copenhagen, Denmark.No affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7513171

Citation

Andersen, C B., et al. "Acute Kidney Graft Rejection. a Morphological and Immunohistological Study On "zero-hour" and Follow-up Biopsies With Special Emphasis On Cellular Infiltrates and Adhesion Molecules." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 102, no. 1, 1994, pp. 23-37.
Andersen CB, Ladefoged SD, Larsen S. Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. APMIS. 1994;102(1):23-37.
Andersen, C. B., Ladefoged, S. D., & Larsen, S. (1994). Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 102(1), 23-37.
Andersen CB, Ladefoged SD, Larsen S. Acute Kidney Graft Rejection. a Morphological and Immunohistological Study On "zero-hour" and Follow-up Biopsies With Special Emphasis On Cellular Infiltrates and Adhesion Molecules. APMIS. 1994;102(1):23-37. PubMed PMID: 7513171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. AU - Andersen,C B, AU - Ladefoged,S D, AU - Larsen,S, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 23 EP - 37 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 102 IS - 1 N2 - Serial biopsies from 41 consecutive renal allotransplanted patients were evaluated in order to obtain pretransplant data as well as information on well-functioning and acutely rejecting grafts. Each patient served as his own control. Thirty-five patients were followed according to the schedule which included biopsy prior to transplantation, shortly after opening of reanastomosis, at least once postoperatively (days 7-10), and furthermore whenever clinically indicated. The morphological evaluation was in each case combined with immunofluorescence (to detect immunoglobulins and complement fractions) and immunohistochemistry with a wide panel of monoclonal antibodies for T cells (CD2, CD3, CD4, CD8, gamma delta), B cells (CD20, CD22), macrophages (CD68, MAC387) NK cells (leu-7, CD16), activation markers (IL-2-R, Ki-67, transferrin-R), MHC antigens (HLA-ABC, HLA-DR), adhesion molecules (ICAM-1, VCAM-1, ELAM-1, PADGEM, VLA-4, LFA-1 alpha/beta), and growth factors (EGF, TGF-alpha, EGF-R). When 132 biopsies and 10 failed allografts were examined, no specific morphological or immunohistological parameter predictive of rejection or graft outcome could be found. Morphology in follow-up biopsies from non-rejecting and rejecting patients revealed a continuum of inflammatory changes, and several non-rejecting cases demonstrated cellular inflammatory infiltrates which could not be discriminated from those seen in acute rejection. Of the patients 44% had acute rejection accompanied by increased infiltration of T cells and macrophages showing enhanced IL-2-R expression, increased tubular and endothelial staining for MHC class II, ICAM-1, and VCAM-1, and strong leukocytic expression of VLA-4 and LFA-1 alpha/beta. SN - 0903-4641 UR - https://www.unboundmedicine.com/medline/citation/7513171/Acute_kidney_graft_rejection__A_morphological_and_immunohistological_study_on_"zero_hour"_and_follow_up_biopsies_with_special_emphasis_on_cellular_infiltrates_and_adhesion_molecules_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0903-4641&date=1994&volume=102&spage=23 DB - PRIME DP - Unbound Medicine ER -