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Vaccination with T cell receptor peptides primes anti-receptor cytotoxic T lymphocytes (CTL) and anergizes T cells specifically recognized by these CTL.
Eur J Immunol. 1994 May; 24(5):1172-80.EJ

Abstract

We selected three peptides from the germ-line sequence of the V beta 8.2 and J beta 2.3 gene segments of the murine T cell receptor for antigen (TCR) which contained putative Kd- and Ld-restricted epitopes. Immunization of BALB/c (H-2d) mice with the V beta 8.2(67-90) 23-mer peptide 1 as well as the 15-mer V beta 8.2(95-108)-peptide 2 efficiently primed specific CD8+ cytotoxic T lymphocyte (CTL) responses in vivo against natural TCR-V beta 8.2 epitopes. V beta 8.2+ T cells were not deleted in TCR peptide-immunized mice because the fractions of V beta 8.2+ CD4+ and V beta 8.2+ CD8+ T cells in spleen and lymph nodes were not altered. The proliferative response of V beta 8.2+ T cells to stimulation by monoclonal antibody F23.2 was selectively suppressed (by 60-80%) in peptide-immunized BALB/c mice, indicating partial anergy of this T subset. Immunization of BALB/c mice with the J beta 2.3-derived peptide 3 stimulated a CD8+ CTL response against a class I-restricted epitope within this J beta segment that was also generated during natural "endogenous" processing of this self antigen. These data confirm the predictive value of major histocompatibility complex class I allele-specific motifs. The described experiments indicate that TCR peptide-primed CD8+ CTL recognize class I-restricted, natural V beta/J beta-TCR epitopes. Such anti-TCR CTL may, thus, operate in V beta-specific immunoregulation of the T cell system suppressing their functional reactivity without deleting them.

Authors+Show Affiliations

Institute of Microbiology, University of Ulm, FRG.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7514132

Citation

Kuhröber, A, et al. "Vaccination With T Cell Receptor Peptides Primes Anti-receptor Cytotoxic T Lymphocytes (CTL) and Anergizes T Cells Specifically Recognized By These CTL." European Journal of Immunology, vol. 24, no. 5, 1994, pp. 1172-80.
Kuhröber A, Schirmbeck R, Reimann J. Vaccination with T cell receptor peptides primes anti-receptor cytotoxic T lymphocytes (CTL) and anergizes T cells specifically recognized by these CTL. Eur J Immunol. 1994;24(5):1172-80.
Kuhröber, A., Schirmbeck, R., & Reimann, J. (1994). Vaccination with T cell receptor peptides primes anti-receptor cytotoxic T lymphocytes (CTL) and anergizes T cells specifically recognized by these CTL. European Journal of Immunology, 24(5), 1172-80.
Kuhröber A, Schirmbeck R, Reimann J. Vaccination With T Cell Receptor Peptides Primes Anti-receptor Cytotoxic T Lymphocytes (CTL) and Anergizes T Cells Specifically Recognized By These CTL. Eur J Immunol. 1994;24(5):1172-80. PubMed PMID: 7514132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vaccination with T cell receptor peptides primes anti-receptor cytotoxic T lymphocytes (CTL) and anergizes T cells specifically recognized by these CTL. AU - Kuhröber,A, AU - Schirmbeck,R, AU - Reimann,J, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 1172 EP - 80 JF - European journal of immunology JO - Eur. J. Immunol. VL - 24 IS - 5 N2 - We selected three peptides from the germ-line sequence of the V beta 8.2 and J beta 2.3 gene segments of the murine T cell receptor for antigen (TCR) which contained putative Kd- and Ld-restricted epitopes. Immunization of BALB/c (H-2d) mice with the V beta 8.2(67-90) 23-mer peptide 1 as well as the 15-mer V beta 8.2(95-108)-peptide 2 efficiently primed specific CD8+ cytotoxic T lymphocyte (CTL) responses in vivo against natural TCR-V beta 8.2 epitopes. V beta 8.2+ T cells were not deleted in TCR peptide-immunized mice because the fractions of V beta 8.2+ CD4+ and V beta 8.2+ CD8+ T cells in spleen and lymph nodes were not altered. The proliferative response of V beta 8.2+ T cells to stimulation by monoclonal antibody F23.2 was selectively suppressed (by 60-80%) in peptide-immunized BALB/c mice, indicating partial anergy of this T subset. Immunization of BALB/c mice with the J beta 2.3-derived peptide 3 stimulated a CD8+ CTL response against a class I-restricted epitope within this J beta segment that was also generated during natural "endogenous" processing of this self antigen. These data confirm the predictive value of major histocompatibility complex class I allele-specific motifs. The described experiments indicate that TCR peptide-primed CD8+ CTL recognize class I-restricted, natural V beta/J beta-TCR epitopes. Such anti-TCR CTL may, thus, operate in V beta-specific immunoregulation of the T cell system suppressing their functional reactivity without deleting them. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/7514132/Vaccination_with_T_cell_receptor_peptides_primes_anti_receptor_cytotoxic_T_lymphocytes__CTL__and_anergizes_T_cells_specifically_recognized_by_these_CTL_ L2 - https://doi.org/10.1002/eji.1830240525 DB - PRIME DP - Unbound Medicine ER -