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Increased Na+/K(+)-pump activity and adenosine triphosphate utilization after compound 48/80-induced histamine secretion from rat mast cells.
Biochem Pharmacol. 1994 May 18; 47(10):1731-6.BP

Abstract

The Na+/K(+)-pump activity and the utilization of adenosine triphosphate (ATP) were studied in rat peritoneal mast cells after histamine secretion induced by compound 48/80. We measured the ouabain-sensitive K(+)-uptake by a radioactive technique (86Rb+). The ATP content and the glycolytic ATP-production were measured by the bioluminescence technique (firefly lantern) and by measurement of the lactate production under anaerobic conditions (antimycin A, oligomycin), respectively. There was an increased requirement for ATP after the secretory response associated with an increased activity of the Na+/K(+)-pump. The anaerobic, but not the aerobic, pathway for ATP-synthesis was able to respond to the increased ATP-requirement. The ATP-requirement of the Na+/K(+)-pump was only partly satisfied when ATP was supplied from either the glycolytic or the oxidative pathway. This may indicate that the availability of ATP was the limiting factor for the activity of the Na+/K(+)-pump following histamine secretion under these conditions. It is concluded that the large increase in Na+/K(+)-pump activity after a secretory response is a likely explanation for the long lasting ATP-decrease in mast cells that follows histamine secretion.

Authors+Show Affiliations

Department of Pharmacology, Odense University, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7515620

Citation

Johansen, T, and H Praetorius. "Increased Na+/K(+)-pump Activity and Adenosine Triphosphate Utilization After Compound 48/80-induced Histamine Secretion From Rat Mast Cells." Biochemical Pharmacology, vol. 47, no. 10, 1994, pp. 1731-6.
Johansen T, Praetorius H. Increased Na+/K(+)-pump activity and adenosine triphosphate utilization after compound 48/80-induced histamine secretion from rat mast cells. Biochem Pharmacol. 1994;47(10):1731-6.
Johansen, T., & Praetorius, H. (1994). Increased Na+/K(+)-pump activity and adenosine triphosphate utilization after compound 48/80-induced histamine secretion from rat mast cells. Biochemical Pharmacology, 47(10), 1731-6.
Johansen T, Praetorius H. Increased Na+/K(+)-pump Activity and Adenosine Triphosphate Utilization After Compound 48/80-induced Histamine Secretion From Rat Mast Cells. Biochem Pharmacol. 1994 May 18;47(10):1731-6. PubMed PMID: 7515620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased Na+/K(+)-pump activity and adenosine triphosphate utilization after compound 48/80-induced histamine secretion from rat mast cells. AU - Johansen,T, AU - Praetorius,H, PY - 1994/5/18/pubmed PY - 1994/5/18/medline PY - 1994/5/18/entrez SP - 1731 EP - 6 JF - Biochemical pharmacology JO - Biochem Pharmacol VL - 47 IS - 10 N2 - The Na+/K(+)-pump activity and the utilization of adenosine triphosphate (ATP) were studied in rat peritoneal mast cells after histamine secretion induced by compound 48/80. We measured the ouabain-sensitive K(+)-uptake by a radioactive technique (86Rb+). The ATP content and the glycolytic ATP-production were measured by the bioluminescence technique (firefly lantern) and by measurement of the lactate production under anaerobic conditions (antimycin A, oligomycin), respectively. There was an increased requirement for ATP after the secretory response associated with an increased activity of the Na+/K(+)-pump. The anaerobic, but not the aerobic, pathway for ATP-synthesis was able to respond to the increased ATP-requirement. The ATP-requirement of the Na+/K(+)-pump was only partly satisfied when ATP was supplied from either the glycolytic or the oxidative pathway. This may indicate that the availability of ATP was the limiting factor for the activity of the Na+/K(+)-pump following histamine secretion under these conditions. It is concluded that the large increase in Na+/K(+)-pump activity after a secretory response is a likely explanation for the long lasting ATP-decrease in mast cells that follows histamine secretion. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/7515620/Increased_Na+/K_+__pump_activity_and_adenosine_triphosphate_utilization_after_compound_48/80_induced_histamine_secretion_from_rat_mast_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-2952(94)90299-2 DB - PRIME DP - Unbound Medicine ER -