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The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients.
Folia Parasitol (Praha). 1993; 40(4):261-6.FP

Abstract

A protocol for the handling of small intestinal biopsies from HIV-infected patients is presented. This protocol includes the Warthin-Starry stain for the detection of microsporidia. This stain has proved a reliable and sensitive diagnostic technique for microsporidial infections as it stains both Enterocytozoon bieneusi and Septata intestinalis in duodenal enterocytes. Because the stain demonstrates Septata intestinalis in lamina propria macrophages as well as enterocytes, it allows for the practical differentiation of these two microsporidial infections. The Warthin-Starry stain has also demonstrated Septata intestinalis in nasal and colonic biopsies in some of these patients. Since the completion of an earlier study, a further 40 cases of Enterocytozoon bieneusi and three cases of Septata intestinalis have been diagnosed in just over 240 consecutive duodenal biopsies from HIV positive patients presenting with diarrhoea and other gastrointestinal complaints. Other opportunistic infections include cytomegalovirus in four cases, mycobacteria in eight cases, cryptosporidia in nine cases, giardia in four cases and Isospora belli in one case. Since the ratio of these opportunistic infections has remained much the same as in the previous study of 180 consecutive duodenal biopsies, we suggest that these rates may reflect the actual prevalence of microsporidial infections in AIDS patients in Sydney, Australia.

Authors+Show Affiliations

Department of Anatomical Pathology, St. Vincent's Hospital, Darlinghurst, Sydney, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7516907

Citation

Field, A S., et al. "The Warthin-Starry Stain in the Diagnosis of Small Intestinal Microsporidiosis in HIV-infected Patients." Folia Parasitologica, vol. 40, no. 4, 1993, pp. 261-6.
Field AS, Marriott DJ, Hing MC. The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients. Folia Parasitol (Praha). 1993;40(4):261-6.
Field, A. S., Marriott, D. J., & Hing, M. C. (1993). The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients. Folia Parasitologica, 40(4), 261-6.
Field AS, Marriott DJ, Hing MC. The Warthin-Starry Stain in the Diagnosis of Small Intestinal Microsporidiosis in HIV-infected Patients. Folia Parasitol (Praha). 1993;40(4):261-6. PubMed PMID: 7516907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients. AU - Field,A S, AU - Marriott,D J, AU - Hing,M C, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 261 EP - 6 JF - Folia parasitologica JO - Folia Parasitol (Praha) VL - 40 IS - 4 N2 - A protocol for the handling of small intestinal biopsies from HIV-infected patients is presented. This protocol includes the Warthin-Starry stain for the detection of microsporidia. This stain has proved a reliable and sensitive diagnostic technique for microsporidial infections as it stains both Enterocytozoon bieneusi and Septata intestinalis in duodenal enterocytes. Because the stain demonstrates Septata intestinalis in lamina propria macrophages as well as enterocytes, it allows for the practical differentiation of these two microsporidial infections. The Warthin-Starry stain has also demonstrated Septata intestinalis in nasal and colonic biopsies in some of these patients. Since the completion of an earlier study, a further 40 cases of Enterocytozoon bieneusi and three cases of Septata intestinalis have been diagnosed in just over 240 consecutive duodenal biopsies from HIV positive patients presenting with diarrhoea and other gastrointestinal complaints. Other opportunistic infections include cytomegalovirus in four cases, mycobacteria in eight cases, cryptosporidia in nine cases, giardia in four cases and Isospora belli in one case. Since the ratio of these opportunistic infections has remained much the same as in the previous study of 180 consecutive duodenal biopsies, we suggest that these rates may reflect the actual prevalence of microsporidial infections in AIDS patients in Sydney, Australia. SN - 0015-5683 UR - https://www.unboundmedicine.com/medline/citation/7516907/The_Warthin_Starry_stain_in_the_diagnosis_of_small_intestinal_microsporidiosis_in_HIV_infected_patients_ L2 - https://folia.paru.cas.cz/link/1993/4/261.html DB - PRIME DP - Unbound Medicine ER -