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Construction of a specific DNA probe for diagnosis of melioidosis and use as an epidemiological marker of Pseudomonas pseudomallei.
Mol Cell Probes. 1994 Feb; 8(1):1-9.MC

Abstract

Pseudomonas pseudomallei is a causative agent of melioidosis. The disease manifestations range from fulminant sepsis to asymptomatic seroconversion. In septicemic cases, a mortality rate of 80-90% is reported. Rapid and specific diagnosis has become important to the clinical microbiology laboratory. We have developed a P. pseudomallei-specific DNA probe. The cloned fragment, herein designated pKKU-S23L, contained 1.5 kb of P. pseudomallei chromosomal DNA. A radioactively labelled pKKU-S23L insert could detect 1.5 ng of its genomic DNA or 40,000 P. pseudomallei cells. The probe was highly specific for P. pseudomallei DNA and did not cross-hybridize with DNAs prepared from other related bacteria. Using pKKU-S23L as a probe in total cellular DNA digestions and Southern blot hybridization, we were able to classify 60 P. pseudomallei clinical isolates obtained from individual melioidosis patients into eight categories. Therefore, this probe has a potential not only for use in development of specific detection of bacterial DNA in clinical specimens but also for application in epidemiological studies of P. pseudomallei.

Authors+Show Affiliations

Department of Biotechnology, Faculty of Technology, Khon Kaen University, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7518036

Citation

Sermswan, R W., et al. "Construction of a Specific DNA Probe for Diagnosis of Melioidosis and Use as an Epidemiological Marker of Pseudomonas Pseudomallei." Molecular and Cellular Probes, vol. 8, no. 1, 1994, pp. 1-9.
Sermswan RW, Wongratanacheewin S, Tattawasart U, et al. Construction of a specific DNA probe for diagnosis of melioidosis and use as an epidemiological marker of Pseudomonas pseudomallei. Mol Cell Probes. 1994;8(1):1-9.
Sermswan, R. W., Wongratanacheewin, S., Tattawasart, U., & Wongwajana, S. (1994). Construction of a specific DNA probe for diagnosis of melioidosis and use as an epidemiological marker of Pseudomonas pseudomallei. Molecular and Cellular Probes, 8(1), 1-9.
Sermswan RW, et al. Construction of a Specific DNA Probe for Diagnosis of Melioidosis and Use as an Epidemiological Marker of Pseudomonas Pseudomallei. Mol Cell Probes. 1994;8(1):1-9. PubMed PMID: 7518036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a specific DNA probe for diagnosis of melioidosis and use as an epidemiological marker of Pseudomonas pseudomallei. AU - Sermswan,R W, AU - Wongratanacheewin,S, AU - Tattawasart,U, AU - Wongwajana,S, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 1 EP - 9 JF - Molecular and cellular probes JO - Mol Cell Probes VL - 8 IS - 1 N2 - Pseudomonas pseudomallei is a causative agent of melioidosis. The disease manifestations range from fulminant sepsis to asymptomatic seroconversion. In septicemic cases, a mortality rate of 80-90% is reported. Rapid and specific diagnosis has become important to the clinical microbiology laboratory. We have developed a P. pseudomallei-specific DNA probe. The cloned fragment, herein designated pKKU-S23L, contained 1.5 kb of P. pseudomallei chromosomal DNA. A radioactively labelled pKKU-S23L insert could detect 1.5 ng of its genomic DNA or 40,000 P. pseudomallei cells. The probe was highly specific for P. pseudomallei DNA and did not cross-hybridize with DNAs prepared from other related bacteria. Using pKKU-S23L as a probe in total cellular DNA digestions and Southern blot hybridization, we were able to classify 60 P. pseudomallei clinical isolates obtained from individual melioidosis patients into eight categories. Therefore, this probe has a potential not only for use in development of specific detection of bacterial DNA in clinical specimens but also for application in epidemiological studies of P. pseudomallei. SN - 0890-8508 UR - https://www.unboundmedicine.com/medline/citation/7518036/Construction_of_a_specific_DNA_probe_for_diagnosis_of_melioidosis_and_use_as_an_epidemiological_marker_of_Pseudomonas_pseudomallei_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-8508(84)71001-2 DB - PRIME DP - Unbound Medicine ER -