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Conformation of the central, three-helix junction of the 5 S ribosomal RNA of Sulfolobus acidocaldarius.
J Mol Biol. 1994 Aug 19; 241(3):415-30.JM

Abstract

The current investigation has focused on the structure of the central, three-helix junction of the 5 S ribosomal RNA from Sulfolobus acidocaldarius, a thermoacidophilic archaebacterium. The 5 S molecule from S. acidocaldarius represents a paradigm for the study of the 5 S junction (and branched RNA structures in general) due to its unusually high degree of predicted secondary structure and stability. In order to study the junction in isolation, a set of three RNA heteroduplex molecules was assembled in which pairs of helices bounding the junction were extended by 70 base-pairs per helix. Since the extended helices comprise more than 95% of the heteroduplex structure, the relative solution conformations of the heteroduplex molecules, in effect, "report" the interhelix (branch) angles bounded by the extended pairs of helices. Examination of these heteroduplex molecules, using a combination of gel electrophoresis and transient electric birefringence measurements, has revealed a tertiary structure for the central branch in which helices I and V are essentially collinear, and in which helix II is relatively free to reorient with respect to the I-V axis.

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Authors+Show Affiliations

Shen Z
Department of Biochemistry, Biophysics and Genetics University of Colorado Health Sciences Center, Denver 80262.
Hagerman PJ
No affiliation info available

MeSH

Base SequenceBirefringenceMolecular Sequence DataMolecular StructureNucleic Acid ConformationRNA, BacterialRNA, Double-StrandedRNA, Ribosomal, 5SSequence Analysis, RNASulfolobus acidocaldarius

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7520504

Citation

Shen, Z, and P J. Hagerman. "Conformation of the Central, Three-helix Junction of the 5 S Ribosomal RNA of Sulfolobus Acidocaldarius." Journal of Molecular Biology, vol. 241, no. 3, 1994, pp. 415-30.
Shen Z, Hagerman PJ. Conformation of the central, three-helix junction of the 5 S ribosomal RNA of Sulfolobus acidocaldarius. J Mol Biol. 1994;241(3):415-30.
Shen, Z., & Hagerman, P. J. (1994). Conformation of the central, three-helix junction of the 5 S ribosomal RNA of Sulfolobus acidocaldarius. Journal of Molecular Biology, 241(3), 415-30.
Shen Z, Hagerman PJ. Conformation of the Central, Three-helix Junction of the 5 S Ribosomal RNA of Sulfolobus Acidocaldarius. J Mol Biol. 1994 Aug 19;241(3):415-30. PubMed PMID: 7520504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conformation of the central, three-helix junction of the 5 S ribosomal RNA of Sulfolobus acidocaldarius. AU - Shen,Z, AU - Hagerman,P J, PY - 1994/8/19/pubmed PY - 1994/8/19/medline PY - 1994/8/19/entrez SP - 415 EP - 30 JF - Journal of molecular biology JO - J Mol Biol VL - 241 IS - 3 N2 - The current investigation has focused on the structure of the central, three-helix junction of the 5 S ribosomal RNA from Sulfolobus acidocaldarius, a thermoacidophilic archaebacterium. The 5 S molecule from S. acidocaldarius represents a paradigm for the study of the 5 S junction (and branched RNA structures in general) due to its unusually high degree of predicted secondary structure and stability. In order to study the junction in isolation, a set of three RNA heteroduplex molecules was assembled in which pairs of helices bounding the junction were extended by 70 base-pairs per helix. Since the extended helices comprise more than 95% of the heteroduplex structure, the relative solution conformations of the heteroduplex molecules, in effect, "report" the interhelix (branch) angles bounded by the extended pairs of helices. Examination of these heteroduplex molecules, using a combination of gel electrophoresis and transient electric birefringence measurements, has revealed a tertiary structure for the central branch in which helices I and V are essentially collinear, and in which helix II is relatively free to reorient with respect to the I-V axis. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/7520504/Conformation_of_the_central_three_helix_junction_of_the_5_S_ribosomal_RNA_of_Sulfolobus_acidocaldarius_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(84)71517-8 DB - PRIME DP - Unbound Medicine ER -