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Effect of abasic sites on bacteriophage T7 protein synthesis.
Mutat Res. 1994 Sep; 325(1):39-45.MR

Abstract

We have examined protein synthesis directed by bacteriophage T7 which had been alkylated with methyl methanesulfonate so as to produce apurinic sites in its DNA in vivo. Both repair-proficient and repair-deficient (xth nfo mutant) strains of Escherichia coli served as host cells. In repair-proficient cells, all three classes of phage proteins were synthesized, although with significant delays. In mutant cells, only class I proteins were produced and their synthesis was delayed and reduced, demonstrating a perturbation of protein synthesis and providing the first in vivo indication that transcription is inhibited by abasic sites. However, the proposed effects of abasic sites on transcription appear to be weaker than those on replication.

Authors+Show Affiliations

Département de biochimie, Université de Montréal, Quebec, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7521011

Citation

Sanchez, G, et al. "Effect of Abasic Sites On Bacteriophage T7 Protein Synthesis." Mutation Research, vol. 325, no. 1, 1994, pp. 39-45.
Sanchez G, Racine JF, Mamet-Bratley MD. Effect of abasic sites on bacteriophage T7 protein synthesis. Mutat Res. 1994;325(1):39-45.
Sanchez, G., Racine, J. F., & Mamet-Bratley, M. D. (1994). Effect of abasic sites on bacteriophage T7 protein synthesis. Mutation Research, 325(1), 39-45.
Sanchez G, Racine JF, Mamet-Bratley MD. Effect of Abasic Sites On Bacteriophage T7 Protein Synthesis. Mutat Res. 1994;325(1):39-45. PubMed PMID: 7521011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of abasic sites on bacteriophage T7 protein synthesis. AU - Sanchez,G, AU - Racine,J F, AU - Mamet-Bratley,M D, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 39 EP - 45 JF - Mutation research JO - Mutat. Res. VL - 325 IS - 1 N2 - We have examined protein synthesis directed by bacteriophage T7 which had been alkylated with methyl methanesulfonate so as to produce apurinic sites in its DNA in vivo. Both repair-proficient and repair-deficient (xth nfo mutant) strains of Escherichia coli served as host cells. In repair-proficient cells, all three classes of phage proteins were synthesized, although with significant delays. In mutant cells, only class I proteins were produced and their synthesis was delayed and reduced, demonstrating a perturbation of protein synthesis and providing the first in vivo indication that transcription is inhibited by abasic sites. However, the proposed effects of abasic sites on transcription appear to be weaker than those on replication. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/7521011/Effect_of_abasic_sites_on_bacteriophage_T7_protein_synthesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0165-7992(94)90025-6 DB - PRIME DP - Unbound Medicine ER -