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Pneumocystis carinii: recognition of the infection in albino rats using different stains.
J Egypt Soc Parasitol. 1994 Aug; 24(2):285-94.JE

Abstract

Pneumocystis carinii is a commensal protozoan which may cause pneumonia in hosts with compromised immune status and may end fatally. Since effective management of pneumocystic pneumonia depends on rapid and accurate recognition of the disease, so, the present study aims to throw lights on the best staining method for cytodiagnosis of this organism with the sequential pathological changes in the infected lungs. An immunosuppression state was induced in albino rats for 6-8 weeks then rats were sacrificed weekly & lungs were examined for infection using different stains. In stained smears, intracystic bodies have been identified using Giemsa, Papanicolaou & Toluidine blue stains. On the other hand, cysts were inspected in paraffin sections using Gomori methenamine silver nitrate (GMS) & weight gram stains. Histopathologically, in sections stained with H & E, features of Pneumocystis carinii pneumonia were obvious, foamy exudation, inflammatory infiltration formed mainly of histiocytes, plasma cells, lymphocytes, thickening of alveolar septa and lastly formation of hyaline membrane in some alveoli.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine, Alexandria University, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7521380

Citation

el-Nassery, S M., et al. "Pneumocystis Carinii: Recognition of the Infection in Albino Rats Using Different Stains." Journal of the Egyptian Society of Parasitology, vol. 24, no. 2, 1994, pp. 285-94.
el-Nassery SM, Rahmy AE, el Gebaly WM, et al. Pneumocystis carinii: recognition of the infection in albino rats using different stains. J Egypt Soc Parasitol. 1994;24(2):285-94.
el-Nassery, S. M., Rahmy, A. E., el Gebaly, W. M., & Sadaka, H. A. (1994). Pneumocystis carinii: recognition of the infection in albino rats using different stains. Journal of the Egyptian Society of Parasitology, 24(2), 285-94.
el-Nassery SM, et al. Pneumocystis Carinii: Recognition of the Infection in Albino Rats Using Different Stains. J Egypt Soc Parasitol. 1994;24(2):285-94. PubMed PMID: 7521380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pneumocystis carinii: recognition of the infection in albino rats using different stains. AU - el-Nassery,S M, AU - Rahmy,A E, AU - el Gebaly,W M, AU - Sadaka,H A, PY - 1994/8/1/pubmed PY - 1994/8/1/medline PY - 1994/8/1/entrez SP - 285 EP - 94 JF - Journal of the Egyptian Society of Parasitology JO - J Egypt Soc Parasitol VL - 24 IS - 2 N2 - Pneumocystis carinii is a commensal protozoan which may cause pneumonia in hosts with compromised immune status and may end fatally. Since effective management of pneumocystic pneumonia depends on rapid and accurate recognition of the disease, so, the present study aims to throw lights on the best staining method for cytodiagnosis of this organism with the sequential pathological changes in the infected lungs. An immunosuppression state was induced in albino rats for 6-8 weeks then rats were sacrificed weekly & lungs were examined for infection using different stains. In stained smears, intracystic bodies have been identified using Giemsa, Papanicolaou & Toluidine blue stains. On the other hand, cysts were inspected in paraffin sections using Gomori methenamine silver nitrate (GMS) & weight gram stains. Histopathologically, in sections stained with H & E, features of Pneumocystis carinii pneumonia were obvious, foamy exudation, inflammatory infiltration formed mainly of histiocytes, plasma cells, lymphocytes, thickening of alveolar septa and lastly formation of hyaline membrane in some alveoli. SN - 1110-0583 UR - https://www.unboundmedicine.com/medline/citation/7521380/Pneumocystis_carinii:_recognition_of_the_infection_in_albino_rats_using_different_stains_ DB - PRIME DP - Unbound Medicine ER -