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The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene.
EMBO J. 1994 Sep 15; 13(18):4382-9.EJ

Abstract

The HOG signal pathway of the yeast Saccharomyces cerevisiae is defined by the PBS2 and HOG1 genes encoding members of the MAP kinase kinase and of the MAP kinase family, respectively. Mutations in this pathway (deletions of PBS2 or HOG1, or point mutations in HOG1) almost completely abolish the induction of transcription by osmotic stress that is mediated by stress response elements (STREs). We have demonstrated previously that STREs also mediate induction of transcription by heat shock, nitrogen starvation and oxidative stress. This study shows that they are also activated by low external pH, sorbate, benzoate or ethanol stress. Induction by these other stress signals appears to be HOG pathway independent. HOG1-dependent osmotic induction of transcription of the CTT1 gene encoding the cytosolic catalase T occurs in the presence of a protein synthesis inhibitor and can be detected rapidly after an increase of tyrosine phosphorylation of Hog1p triggered by high osmolarity. Consistent with a role of STREs in the induction of stress resistance, a number of other stress protein genes (e.g. HSP104) are regulated like CTT1. Furthermore, catalase T was shown to be important for viability under severe osmotic stress, and heat shock was demonstrated to provide cross-protection against osmotic stress.

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Authors+Show Affiliations

Schüller C
Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
Brewster JL
No affiliation info available
Alexander MR
No affiliation info available
Gustin MC
No affiliation info available
Ruis H
No affiliation info available

MeSH

Adaptation, PhysiologicalCalcium-Calmodulin-Dependent Protein KinasesCatalaseCyclic AMP-Dependent Protein KinasesGene Expression Regulation, FungalGenes, FungalMitogen-Activated Protein Kinase KinasesMitogen-Activated Protein KinasesMolecular Sequence DataOsmotic PressurePhosphotyrosineProtein KinasesRNA, MessengerRegulatory Sequences, Nucleic AcidSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSignal TransductionTranscription, GeneticTyrosine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7523111

Citation

Schüller, C, et al. "The HOG Pathway Controls Osmotic Regulation of Transcription Via the Stress Response Element (STRE) of the Saccharomyces Cerevisiae CTT1 Gene." The EMBO Journal, vol. 13, no. 18, 1994, pp. 4382-9.
Schüller C, Brewster JL, Alexander MR, et al. The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J. 1994;13(18):4382-9.
Schüller, C., Brewster, J. L., Alexander, M. R., Gustin, M. C., & Ruis, H. (1994). The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. The EMBO Journal, 13(18), 4382-9.
Schüller C, et al. The HOG Pathway Controls Osmotic Regulation of Transcription Via the Stress Response Element (STRE) of the Saccharomyces Cerevisiae CTT1 Gene. EMBO J. 1994 Sep 15;13(18):4382-9. PubMed PMID: 7523111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. AU - Schüller,C, AU - Brewster,J L, AU - Alexander,M R, AU - Gustin,M C, AU - Ruis,H, PY - 1994/9/15/pubmed PY - 1994/9/15/medline PY - 1994/9/15/entrez SP - 4382 EP - 9 JF - The EMBO journal JO - EMBO J VL - 13 IS - 18 N2 - The HOG signal pathway of the yeast Saccharomyces cerevisiae is defined by the PBS2 and HOG1 genes encoding members of the MAP kinase kinase and of the MAP kinase family, respectively. Mutations in this pathway (deletions of PBS2 or HOG1, or point mutations in HOG1) almost completely abolish the induction of transcription by osmotic stress that is mediated by stress response elements (STREs). We have demonstrated previously that STREs also mediate induction of transcription by heat shock, nitrogen starvation and oxidative stress. This study shows that they are also activated by low external pH, sorbate, benzoate or ethanol stress. Induction by these other stress signals appears to be HOG pathway independent. HOG1-dependent osmotic induction of transcription of the CTT1 gene encoding the cytosolic catalase T occurs in the presence of a protein synthesis inhibitor and can be detected rapidly after an increase of tyrosine phosphorylation of Hog1p triggered by high osmolarity. Consistent with a role of STREs in the induction of stress resistance, a number of other stress protein genes (e.g. HSP104) are regulated like CTT1. Furthermore, catalase T was shown to be important for viability under severe osmotic stress, and heat shock was demonstrated to provide cross-protection against osmotic stress. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/7523111/The_HOG_pathway_controls_osmotic_regulation_of_transcription_via_the_stress_response_element__STRE__of_the_Saccharomyces_cerevisiae_CTT1_gene_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1994&volume=13&issue=18&spage=4382 DB - PRIME DP - Unbound Medicine ER -