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PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids.
Mol Microbiol 1993; 9(6):1131-42MM

Abstract

The replication of ColE1-related plasmids is controlled by an unstable antisense RNA, RNAI, which can interfere with the successful processing of the RNAII primer of replication. We show here that a host protein, PcnB, supports replication by promoting the decay of RNAI. In bacterial strains deleted for PcnB a stable, active form of RNAI, RNAI*, which appears to be identical to the product of 5'-end processing by RNAase E, accumulates. This leads to a reduction in plasmid copy number. We show, using a GST-PcnB fusion protein, that PcnB does not interfere with RNAI/RNAII binding in vitro. The fusion protein, like PcnB, has polyadenylating activity and is able to polyadenylate RNAI (and also another antisense RNA, CopA) in vitro.

Authors+Show Affiliations

Institute of Cell and Molecular Biology, University of Edinburgh, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7523833

Citation

He, L, et al. "PcnB Is Required for the Rapid Degradation of RNAI, the Antisense RNA That Controls the Copy Number of ColE1-related Plasmids." Molecular Microbiology, vol. 9, no. 6, 1993, pp. 1131-42.
He L, Söderbom F, Wagner EG, et al. PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids. Mol Microbiol. 1993;9(6):1131-42.
He, L., Söderbom, F., Wagner, E. G., Binnie, U., Binns, N., & Masters, M. (1993). PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids. Molecular Microbiology, 9(6), pp. 1131-42.
He L, et al. PcnB Is Required for the Rapid Degradation of RNAI, the Antisense RNA That Controls the Copy Number of ColE1-related Plasmids. Mol Microbiol. 1993;9(6):1131-42. PubMed PMID: 7523833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids. AU - He,L, AU - Söderbom,F, AU - Wagner,E G, AU - Binnie,U, AU - Binns,N, AU - Masters,M, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 1131 EP - 42 JF - Molecular microbiology JO - Mol. Microbiol. VL - 9 IS - 6 N2 - The replication of ColE1-related plasmids is controlled by an unstable antisense RNA, RNAI, which can interfere with the successful processing of the RNAII primer of replication. We show here that a host protein, PcnB, supports replication by promoting the decay of RNAI. In bacterial strains deleted for PcnB a stable, active form of RNAI, RNAI*, which appears to be identical to the product of 5'-end processing by RNAase E, accumulates. This leads to a reduction in plasmid copy number. We show, using a GST-PcnB fusion protein, that PcnB does not interfere with RNAI/RNAII binding in vitro. The fusion protein, like PcnB, has polyadenylating activity and is able to polyadenylate RNAI (and also another antisense RNA, CopA) in vitro. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/7523833/PcnB_is_required_for_the_rapid_degradation_of_RNAI_the_antisense_RNA_that_controls_the_copy_number_of_ColE1_related_plasmids_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1993&volume=9&issue=6&spage=1131 DB - PRIME DP - Unbound Medicine ER -