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Differential replication of plasmids during stringent and relaxed response of Escherichia coli.
Plasmid. 1994 Jul; 32(1):89-94.P

Abstract

Stringent control of DNA replication has been demonstrated for a few replicons like oriC, pBR322, and plasmids derived from coliphage lambda. In this study we investigated the replication of other plasmids harboring a well defined origin (orip15A, oripSC101, and oriRK2 = oriV) in amino-acid-starved stringent and relaxed strains of Escherichia coli. We found differential replication of plasmids during stringent and relaxed response. Inhibition of DNA synthesis or amplification of plasmid DNA in amino acid-starved relA+ and relA- cells depends on the kind of replicon and, surprisingly, on the nature of deprived amino acid. We conclude that there are no general rules for stringent control of DNA replication and each replicon must be considered separately. There are, however, possible explanations for the differences shown between replicons in their response to stringent and relaxed conditions.

Authors+Show Affiliations

Department of Molecular Biology, University of Gdańsk, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7527562

Citation

Herman, A, et al. "Differential Replication of Plasmids During Stringent and Relaxed Response of Escherichia Coli." Plasmid, vol. 32, no. 1, 1994, pp. 89-94.
Herman A, Wegrzyn A, Wegrzyn G. Differential replication of plasmids during stringent and relaxed response of Escherichia coli. Plasmid. 1994;32(1):89-94.
Herman, A., Wegrzyn, A., & Wegrzyn, G. (1994). Differential replication of plasmids during stringent and relaxed response of Escherichia coli. Plasmid, 32(1), 89-94.
Herman A, Wegrzyn A, Wegrzyn G. Differential Replication of Plasmids During Stringent and Relaxed Response of Escherichia Coli. Plasmid. 1994;32(1):89-94. PubMed PMID: 7527562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential replication of plasmids during stringent and relaxed response of Escherichia coli. AU - Herman,A, AU - Wegrzyn,A, AU - Wegrzyn,G, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 89 EP - 94 JF - Plasmid JO - Plasmid VL - 32 IS - 1 N2 - Stringent control of DNA replication has been demonstrated for a few replicons like oriC, pBR322, and plasmids derived from coliphage lambda. In this study we investigated the replication of other plasmids harboring a well defined origin (orip15A, oripSC101, and oriRK2 = oriV) in amino-acid-starved stringent and relaxed strains of Escherichia coli. We found differential replication of plasmids during stringent and relaxed response. Inhibition of DNA synthesis or amplification of plasmid DNA in amino acid-starved relA+ and relA- cells depends on the kind of replicon and, surprisingly, on the nature of deprived amino acid. We conclude that there are no general rules for stringent control of DNA replication and each replicon must be considered separately. There are, however, possible explanations for the differences shown between replicons in their response to stringent and relaxed conditions. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/7527562/Differential_replication_of_plasmids_during_stringent_and_relaxed_response_of_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147619X84710493 DB - PRIME DP - Unbound Medicine ER -