Tags

Type your tag names separated by a space and hit enter

Sickle reticulocytes adhere to VCAM-1.
Blood. 1995 Jan 01; 85(1):268-74.Blood

Abstract

Adherence of sickle (SS) erythrocytes to endothelial cells represents interactions between red blood cell (RBC) and endothelial cell surface molecules. To enhance our understanding of the ligands involved, we transfected COS cells with the cDNAs of two endothelial cell adhesion molecules, VCAM-1 and E-selectin, and measured the binding of normal and sickle RBCs after static incubation. The percentage of COS cells with rosettes (five or more adherent RBCs) was determined. Normal RBCs did not adhere to VCAM-1-transfected COS cells. Unfractionated SS RBCs formed rosettes on the VCAM-1-transfected COS cells (mean +/- SD, 5.85% +/- 4.98%). Low-density SS RBCs were more adherent than high-density SS RBCs (P < .005). The adherent SS RBCs were a young reticulocyte population, staining positively for transferrin receptor. Another measure of reticulocyte age, RNA content, also correlated with adherence. SS RBC binding was specific--inhibitable by antibodies to either VCAM-1 or the alpha 4 integrin chain of VLA-4. In contrast, there was no significant adherence of normal or SS RBCs to E-selectin-transfected COS cells. These results suggest that young reticulocytes bind to endothelial cell VCAM-1 via VLA-4 integrin.

Authors+Show Affiliations

Department of Pediatrics, Children's Hospital, Boston, MA 02115.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7528569

Citation

Gee, B E., and O S. Platt. "Sickle Reticulocytes Adhere to VCAM-1." Blood, vol. 85, no. 1, 1995, pp. 268-74.
Gee BE, Platt OS. Sickle reticulocytes adhere to VCAM-1. Blood. 1995;85(1):268-74.
Gee, B. E., & Platt, O. S. (1995). Sickle reticulocytes adhere to VCAM-1. Blood, 85(1), 268-74.
Gee BE, Platt OS. Sickle Reticulocytes Adhere to VCAM-1. Blood. 1995 Jan 1;85(1):268-74. PubMed PMID: 7528569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sickle reticulocytes adhere to VCAM-1. AU - Gee,B E, AU - Platt,O S, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 268 EP - 74 JF - Blood JO - Blood VL - 85 IS - 1 N2 - Adherence of sickle (SS) erythrocytes to endothelial cells represents interactions between red blood cell (RBC) and endothelial cell surface molecules. To enhance our understanding of the ligands involved, we transfected COS cells with the cDNAs of two endothelial cell adhesion molecules, VCAM-1 and E-selectin, and measured the binding of normal and sickle RBCs after static incubation. The percentage of COS cells with rosettes (five or more adherent RBCs) was determined. Normal RBCs did not adhere to VCAM-1-transfected COS cells. Unfractionated SS RBCs formed rosettes on the VCAM-1-transfected COS cells (mean +/- SD, 5.85% +/- 4.98%). Low-density SS RBCs were more adherent than high-density SS RBCs (P < .005). The adherent SS RBCs were a young reticulocyte population, staining positively for transferrin receptor. Another measure of reticulocyte age, RNA content, also correlated with adherence. SS RBC binding was specific--inhibitable by antibodies to either VCAM-1 or the alpha 4 integrin chain of VLA-4. In contrast, there was no significant adherence of normal or SS RBCs to E-selectin-transfected COS cells. These results suggest that young reticulocytes bind to endothelial cell VCAM-1 via VLA-4 integrin. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/7528569/Sickle_reticulocytes_adhere_to_VCAM_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-4971(20)69797-X DB - PRIME DP - Unbound Medicine ER -