TY - JOUR T1 - Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneumocystis carinii in bronchoalveolar lavage specimens. AU - Stager,C E, AU - Fraire,A E, AU - Kim,H S, AU - Estrada,R, AU - Davis,J R, AU - Greenberg,D, AU - Guy,E S, AU - Guntupalli,K K, AU - McBride,R A, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 142 EP - 7 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 119 IS - 2 N2 - Four hundred forty-five bronchoalveolar lavage specimens from patients with the human immunodeficiency virus were preserved in Saccomano's fixative and stained for Pneumocystis carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warrington, Pa) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems Corp, Seattle, Wash). The majority of patients had been treated for suspected P carinii pneumonia for a few days prior to collection of bronchoalveolar lavage specimens. P carinii cysts were detected in 194 (43.6%) specimens. Both stains identified P carinii in 166 (37.3%) specimens and were negative in 251 (56.4%), yielding a concordance rate of 93.7%. P carinii cysts were detected in 25 (5.6%) specimens by the Genetic Systems stain only, and in 3 (0.7%) specimens by the Fungi-Fluor stain only. The sensitivity for detecting cysts of P carinii was significantly greater with the Genetic Systems stain (P < .01). SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/7531427/Modification_of_the_fungi_fluor_and_the_genetic_systems_fluorescent_antibody_methods_for_detection_of_Pneumocystis_carinii_in_bronchoalveolar_lavage_specimens_ L2 - https://medlineplus.gov/hivaids.html DB - PRIME DP - Unbound Medicine ER -