Tags

Type your tag names separated by a space and hit enter

[Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group].
Verh Dtsch Ges Pathol. 1994; 78:214-9.VD

Abstract

Recent cloning of the chromosome breakpoint regions of the reciprocal chromosomal t(11;22) (q24;q12) has revealed that the breakpoints were localized within the EWS gene (Ewings sarcoma gene) on chromosome 22 and the FLI-1 gene on chromosome 11. Thus, molecular genetic techniques were applicable for the detection of this genetic aberration, which occurs as a consistent feature of the Ewings tumor family. By reverse transcription and polymerase chain reaction technique (RT-PCR) in 78% of Ewings sarcoma derived cell lines, and in 91% of primary Ewings tumor tissue t(11;22) specific EWS/FLI-1 fusion transcripts were detected. Furthermore, in bone marrow samples from an Ewings sarcoma patient contaminating tumor cells could be shown by RT-PCR. Our results indicate that molecular genetic detection of the t(11;22) translocation opens a new modality for the differential diagnosis and the staging of Ewings tumor patients.

Authors+Show Affiliations

Dockhorn-Dworniczak B
Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität, Münster.
Schäfer KL
No affiliation info available
Dantcheva R
No affiliation info available
Blasius S
No affiliation info available
van Valen F
No affiliation info available
Burdach S
No affiliation info available
Winkelmann W
No affiliation info available
Jürgens J
No affiliation info available
Böcker W
No affiliation info available

MeSH

Base SequenceBone NeoplasmsCell LineChromosomes, Human, Pair 11Chromosomes, Human, Pair 22Cloning, MolecularDNA PrimersDNA-Binding ProteinsHeterogeneous-Nuclear RibonucleoproteinsHumansMolecular Sequence DataNeoplasm ProteinsNuclear ProteinsOncogene Proteins, FusionPolymerase Chain ReactionProto-Oncogene Protein c-fli-1Proto-Oncogene ProteinsRNA-Binding Protein EWSRNA-Binding ProteinsRibonucleoproteinsSarcoma, EwingTrans-ActivatorsTranslocation, GeneticTumor Cells, Cultured

Pub Type(s)

English Abstract
Journal Article

Language

ger

PubMed ID

7533989

Citation

Dockhorn-Dworniczak, B, et al. "[Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]." Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, vol. 78, 1994, pp. 214-9.
Dockhorn-Dworniczak B, Schäfer KL, Dantcheva R, et al. [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verh Dtsch Ges Pathol. 1994;78:214-9.
Dockhorn-Dworniczak, B., Schäfer, K. L., Dantcheva, R., Blasius, S., van Valen, F., Burdach, S., Winkelmann, W., Jürgens, J., & Böcker, W. (1994). [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, 78, 214-9.
Dockhorn-Dworniczak B, et al. [Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]. Verh Dtsch Ges Pathol. 1994;78:214-9. PubMed PMID: 7533989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. AU - Dockhorn-Dworniczak,B, AU - Schäfer,K L, AU - Dantcheva,R, AU - Blasius,S, AU - van Valen,F, AU - Burdach,S, AU - Winkelmann,W, AU - Jürgens,J, AU - Böcker,W, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 214 EP - 9 JF - Verhandlungen der Deutschen Gesellschaft fur Pathologie JO - Verh Dtsch Ges Pathol VL - 78 N2 - Recent cloning of the chromosome breakpoint regions of the reciprocal chromosomal t(11;22) (q24;q12) has revealed that the breakpoints were localized within the EWS gene (Ewings sarcoma gene) on chromosome 22 and the FLI-1 gene on chromosome 11. Thus, molecular genetic techniques were applicable for the detection of this genetic aberration, which occurs as a consistent feature of the Ewings tumor family. By reverse transcription and polymerase chain reaction technique (RT-PCR) in 78% of Ewings sarcoma derived cell lines, and in 91% of primary Ewings tumor tissue t(11;22) specific EWS/FLI-1 fusion transcripts were detected. Furthermore, in bone marrow samples from an Ewings sarcoma patient contaminating tumor cells could be shown by RT-PCR. Our results indicate that molecular genetic detection of the t(11;22) translocation opens a new modality for the differential diagnosis and the staging of Ewings tumor patients. SN - 0070-4113 UR - https://www.unboundmedicine.com/medline/citation/7533989/[Detection_of_EWS_/FLI_1_gene_fusion_transcripts_by_RT_PCR_as_a_tool_in_the_diagnosis_of_tumors_of_the_Ewing_sarcoma_group]_ L2 - http://www.diseaseinfosearch.org/result/2666 DB - PRIME DP - Unbound Medicine ER -