Citation
Dockhorn-Dworniczak, B, et al. "[Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]." Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, vol. 78, 1994, pp. 214-9.
Dockhorn-Dworniczak B, Schäfer KL, Dantcheva R, et al. [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verh Dtsch Ges Pathol. 1994;78:214-9.
Dockhorn-Dworniczak, B., Schäfer, K. L., Dantcheva, R., Blasius, S., van Valen, F., Burdach, S., Winkelmann, W., Jürgens, J., & Böcker, W. (1994). [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, 78, 214-9.
Dockhorn-Dworniczak B, et al. [Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]. Verh Dtsch Ges Pathol. 1994;78:214-9. PubMed PMID: 7533989.
TY - JOUR
T1 - [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group].
AU - Dockhorn-Dworniczak,B,
AU - Schäfer,K L,
AU - Dantcheva,R,
AU - Blasius,S,
AU - van Valen,F,
AU - Burdach,S,
AU - Winkelmann,W,
AU - Jürgens,J,
AU - Böcker,W,
PY - 1994/1/1/pubmed
PY - 1994/1/1/medline
PY - 1994/1/1/entrez
SP - 214
EP - 9
JF - Verhandlungen der Deutschen Gesellschaft fur Pathologie
JO - Verh Dtsch Ges Pathol
VL - 78
N2 - Recent cloning of the chromosome breakpoint regions of the reciprocal chromosomal t(11;22) (q24;q12) has revealed that the breakpoints were localized within the EWS gene (Ewings sarcoma gene) on chromosome 22 and the FLI-1 gene on chromosome 11. Thus, molecular genetic techniques were applicable for the detection of this genetic aberration, which occurs as a consistent feature of the Ewings tumor family. By reverse transcription and polymerase chain reaction technique (RT-PCR) in 78% of Ewings sarcoma derived cell lines, and in 91% of primary Ewings tumor tissue t(11;22) specific EWS/FLI-1 fusion transcripts were detected. Furthermore, in bone marrow samples from an Ewings sarcoma patient contaminating tumor cells could be shown by RT-PCR. Our results indicate that molecular genetic detection of the t(11;22) translocation opens a new modality for the differential diagnosis and the staging of Ewings tumor patients.
SN - 0070-4113
UR - https://www.unboundmedicine.com/medline/citation/7533989/[Detection_of_EWS_/FLI_1_gene_fusion_transcripts_by_RT_PCR_as_a_tool_in_the_diagnosis_of_tumors_of_the_Ewing_sarcoma_group]_
L2 - http://www.diseaseinfosearch.org/result/2666
DB - PRIME
DP - Unbound Medicine
ER -