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[Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group].
Verh Dtsch Ges Pathol. 1994; 78:214-9.VD

Abstract

Recent cloning of the chromosome breakpoint regions of the reciprocal chromosomal t(11;22) (q24;q12) has revealed that the breakpoints were localized within the EWS gene (Ewings sarcoma gene) on chromosome 22 and the FLI-1 gene on chromosome 11. Thus, molecular genetic techniques were applicable for the detection of this genetic aberration, which occurs as a consistent feature of the Ewings tumor family. By reverse transcription and polymerase chain reaction technique (RT-PCR) in 78% of Ewings sarcoma derived cell lines, and in 91% of primary Ewings tumor tissue t(11;22) specific EWS/FLI-1 fusion transcripts were detected. Furthermore, in bone marrow samples from an Ewings sarcoma patient contaminating tumor cells could be shown by RT-PCR. Our results indicate that molecular genetic detection of the t(11;22) translocation opens a new modality for the differential diagnosis and the staging of Ewings tumor patients.

Authors+Show Affiliations

Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität, Münster.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

ger

PubMed ID

7533989

Citation

Dockhorn-Dworniczak, B, et al. "[Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]." Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, vol. 78, 1994, pp. 214-9.
Dockhorn-Dworniczak B, Schäfer KL, Dantcheva R, et al. [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verh Dtsch Ges Pathol. 1994;78:214-9.
Dockhorn-Dworniczak, B., Schäfer, K. L., Dantcheva, R., Blasius, S., van Valen, F., Burdach, S., Winkelmann, W., Jürgens, J., & Böcker, W. (1994). [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. Verhandlungen Der Deutschen Gesellschaft Fur Pathologie, 78, 214-9.
Dockhorn-Dworniczak B, et al. [Detection of EWS-/FLI-1 Gene Fusion Transcripts By RT-PCR as a Tool in the Diagnosis of Tumors of the Ewing Sarcoma Group]. Verh Dtsch Ges Pathol. 1994;78:214-9. PubMed PMID: 7533989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of EWS-/FLI-1 gene fusion transcripts by RT-PCR as a tool in the diagnosis of tumors of the Ewing sarcoma group]. AU - Dockhorn-Dworniczak,B, AU - Schäfer,K L, AU - Dantcheva,R, AU - Blasius,S, AU - van Valen,F, AU - Burdach,S, AU - Winkelmann,W, AU - Jürgens,J, AU - Böcker,W, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 214 EP - 9 JF - Verhandlungen der Deutschen Gesellschaft fur Pathologie JO - Verh Dtsch Ges Pathol VL - 78 N2 - Recent cloning of the chromosome breakpoint regions of the reciprocal chromosomal t(11;22) (q24;q12) has revealed that the breakpoints were localized within the EWS gene (Ewings sarcoma gene) on chromosome 22 and the FLI-1 gene on chromosome 11. Thus, molecular genetic techniques were applicable for the detection of this genetic aberration, which occurs as a consistent feature of the Ewings tumor family. By reverse transcription and polymerase chain reaction technique (RT-PCR) in 78% of Ewings sarcoma derived cell lines, and in 91% of primary Ewings tumor tissue t(11;22) specific EWS/FLI-1 fusion transcripts were detected. Furthermore, in bone marrow samples from an Ewings sarcoma patient contaminating tumor cells could be shown by RT-PCR. Our results indicate that molecular genetic detection of the t(11;22) translocation opens a new modality for the differential diagnosis and the staging of Ewings tumor patients. SN - 0070-4113 UR - https://www.unboundmedicine.com/medline/citation/7533989/[Detection_of_EWS_/FLI_1_gene_fusion_transcripts_by_RT_PCR_as_a_tool_in_the_diagnosis_of_tumors_of_the_Ewing_sarcoma_group]_ L2 - http://www.diseaseinfosearch.org/result/2666 DB - PRIME DP - Unbound Medicine ER -