Tags

Type your tag names separated by a space and hit enter

Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis.
Cytometry. 1994 Dec 15; 18(4):187-98.C

Abstract

New technology allows highly sensitive flow cytometric detection and quantitative analysis of intracellular antigens in normal and malignant hemopoietic cells. With this technology, the earliest stages of myeloid and lymphoid differentiation can easily and reliably be identified using antibodies directed against (pro-)myeloperoxidase/MPO, CD22 and CD3 antigens, respectively. Particularly for the analysis of undifferentiated acute myeloblastic leukemia (AML) cells, the immunological demonstration of intracellular MPO or its enzymatically inactive proforms is highly relevant, since other myeloid marker molecules such as CD33, CD13, or CDw65 are either not restricted to the granulomonocytic lineage or appear later in differentiation. By combining MPO staining with staining for lactoferrin (LF), undifferentiated cells can be distinguished from the granulomonocytic maturation compartment in bone marrow, since LF is selectively expressed from the myelocyte stage of differentiation onward. The list of informative intracellular antigens to be used in leukemia cell analysis will certainly expand in the near future. One candidate, intracellular CD68, has already been tested by us, and results are presented. Also dealt within this article are surface marker molecules not (as yet) widely used in leukemia cell analysis but with the potential to provide important additional information. Among them are the surface structures CD15, CD15s, CDw65, CD79a (MB-1), CD79b (B29), CD87 (uPA-R), and CD117 (c-kit).

Authors+Show Affiliations

Institut für Immunologie der Universität Wien, Austria.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7534675

Citation

Knapp, W, et al. "Flow Cytometric Analysis of Cell-surface and Intracellular Antigens in Leukemia Diagnosis." Cytometry, vol. 18, no. 4, 1994, pp. 187-98.
Knapp W, Strobl H, Majdic O. Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. Cytometry. 1994;18(4):187-98.
Knapp, W., Strobl, H., & Majdic, O. (1994). Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. Cytometry, 18(4), 187-98.
Knapp W, Strobl H, Majdic O. Flow Cytometric Analysis of Cell-surface and Intracellular Antigens in Leukemia Diagnosis. Cytometry. 1994 Dec 15;18(4):187-98. PubMed PMID: 7534675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. AU - Knapp,W, AU - Strobl,H, AU - Majdic,O, PY - 1994/12/15/pubmed PY - 1994/12/15/medline PY - 1994/12/15/entrez SP - 187 EP - 98 JF - Cytometry JO - Cytometry VL - 18 IS - 4 N2 - New technology allows highly sensitive flow cytometric detection and quantitative analysis of intracellular antigens in normal and malignant hemopoietic cells. With this technology, the earliest stages of myeloid and lymphoid differentiation can easily and reliably be identified using antibodies directed against (pro-)myeloperoxidase/MPO, CD22 and CD3 antigens, respectively. Particularly for the analysis of undifferentiated acute myeloblastic leukemia (AML) cells, the immunological demonstration of intracellular MPO or its enzymatically inactive proforms is highly relevant, since other myeloid marker molecules such as CD33, CD13, or CDw65 are either not restricted to the granulomonocytic lineage or appear later in differentiation. By combining MPO staining with staining for lactoferrin (LF), undifferentiated cells can be distinguished from the granulomonocytic maturation compartment in bone marrow, since LF is selectively expressed from the myelocyte stage of differentiation onward. The list of informative intracellular antigens to be used in leukemia cell analysis will certainly expand in the near future. One candidate, intracellular CD68, has already been tested by us, and results are presented. Also dealt within this article are surface marker molecules not (as yet) widely used in leukemia cell analysis but with the potential to provide important additional information. Among them are the surface structures CD15, CD15s, CDw65, CD79a (MB-1), CD79b (B29), CD87 (uPA-R), and CD117 (c-kit). SN - 0196-4763 UR - https://www.unboundmedicine.com/medline/citation/7534675/Flow_cytometric_analysis_of_cell_surface_and_intracellular_antigens_in_leukemia_diagnosis_ L2 - https://doi.org/10.1002/cyto.990180402 DB - PRIME DP - Unbound Medicine ER -