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B cell antigen receptor-mediated apoptosis. Importance of accessory molecules CD19 and CD22, and of surface IgM cross-linking.
J Immunol. 1995 Apr 01; 154(7):3096-104.JI

Abstract

Engagement of the B cell Ag receptor can induce a suicide pathway in various B cell types. Earlier studies showed that anti-IgM mAb treatment triggers apoptotic death in the Burkitt lymphoma-derived cell line, Ramos. We show that two B cell surface molecules, CD19 and CD22, which have been reported to interact either functionally or structurally with the B cell Ag receptor, also stimulate cell suicide when sufficiently aggregated, both in the Ramos and EBV-infected Ramos AW cell lines. In conditions of lower cross-linking, both molecules enhance the apoptotic response induced by a suboptimal dose of anti-IgM mAb in Ramos cells, reinforcing the notion that CD19 and CD22 may be involved in the death pathway and modulate Ag-induced B cell apoptosis. Similar outcomes were obtained with human tonsillar B cells, which enter the death program upon treatment with cross-linked anti-IgM, -CD19, or -CD22 mAbs. These results indicate that Ag-induced B cell suicide may affect mature B cells in the periphery and may be regulated via the interaction of CD19 and/or CD22 with their respective ligand(s). Early tyrosine phosphorylations were analyzed by Western blotting. The biologic outcome of these various treatments--cell survival or death--could not be related to any detectable new tyrosine-phosphorylated substrate, further questioning the biochemical basis of apoptosis signaling.

Authors+Show Affiliations

INSERM U131, Clamart, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7534787

Citation

Chaouchi, N, et al. "B Cell Antigen Receptor-mediated Apoptosis. Importance of Accessory Molecules CD19 and CD22, and of Surface IgM Cross-linking." Journal of Immunology (Baltimore, Md. : 1950), vol. 154, no. 7, 1995, pp. 3096-104.
Chaouchi N, Vazquez A, Galanaud P, et al. B cell antigen receptor-mediated apoptosis. Importance of accessory molecules CD19 and CD22, and of surface IgM cross-linking. J Immunol. 1995;154(7):3096-104.
Chaouchi, N., Vazquez, A., Galanaud, P., & Leprince, C. (1995). B cell antigen receptor-mediated apoptosis. Importance of accessory molecules CD19 and CD22, and of surface IgM cross-linking. Journal of Immunology (Baltimore, Md. : 1950), 154(7), 3096-104.
Chaouchi N, et al. B Cell Antigen Receptor-mediated Apoptosis. Importance of Accessory Molecules CD19 and CD22, and of Surface IgM Cross-linking. J Immunol. 1995 Apr 1;154(7):3096-104. PubMed PMID: 7534787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - B cell antigen receptor-mediated apoptosis. Importance of accessory molecules CD19 and CD22, and of surface IgM cross-linking. AU - Chaouchi,N, AU - Vazquez,A, AU - Galanaud,P, AU - Leprince,C, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 3096 EP - 104 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 154 IS - 7 N2 - Engagement of the B cell Ag receptor can induce a suicide pathway in various B cell types. Earlier studies showed that anti-IgM mAb treatment triggers apoptotic death in the Burkitt lymphoma-derived cell line, Ramos. We show that two B cell surface molecules, CD19 and CD22, which have been reported to interact either functionally or structurally with the B cell Ag receptor, also stimulate cell suicide when sufficiently aggregated, both in the Ramos and EBV-infected Ramos AW cell lines. In conditions of lower cross-linking, both molecules enhance the apoptotic response induced by a suboptimal dose of anti-IgM mAb in Ramos cells, reinforcing the notion that CD19 and CD22 may be involved in the death pathway and modulate Ag-induced B cell apoptosis. Similar outcomes were obtained with human tonsillar B cells, which enter the death program upon treatment with cross-linked anti-IgM, -CD19, or -CD22 mAbs. These results indicate that Ag-induced B cell suicide may affect mature B cells in the periphery and may be regulated via the interaction of CD19 and/or CD22 with their respective ligand(s). Early tyrosine phosphorylations were analyzed by Western blotting. The biologic outcome of these various treatments--cell survival or death--could not be related to any detectable new tyrosine-phosphorylated substrate, further questioning the biochemical basis of apoptosis signaling. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7534787/B_cell_antigen_receptor_mediated_apoptosis__Importance_of_accessory_molecules_CD19_and_CD22_and_of_surface_IgM_cross_linking_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7534787 DB - PRIME DP - Unbound Medicine ER -