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Humoral immune response against human cytomegalovirus (HCMV)-specific proteins after HCMV infection in lung transplantation as detected with recombinant and naturally occurring proteins.
Clin Diagn Lab Immunol. 1995 Mar; 2(2):214-8.CD

Abstract

The humoral immune response to four intracellularly located cytomegalovirus (CMV) proteins was studied in 15 lung transplant recipients experiencing active CMV infections. Five patients had primary infections, and 10 had secondary infections. Antibodies of the immunoglobulin M (IgM) and IgG classes were measured in an enzyme-linked immunosorbent assay (ELISA) system in which procaryotically expressed recombinant proteins were used as a substrate and also in a monoclonal antibody-based capture ELISA which uses naturally occurring proteins as a substrate. The proteins investigated were the lower matrix protein pp65 (ppUL83), the major DNA-binding protein p52 (ppUL44), and the two immediate early proteins IE1 and IE2 (different splicing products of UL123). Higher levels of antibodies were found to pp65 and especially to p52 than to the immediate early antigens. Antibody levels detected in the recombinant protein-based ELISAs were generally lower than antibody responses detected with the matching antigen capture ELISA. Moreover, some patients appeared to have antibodies mainly to epitopes present on naturally occurring proteins. The antibody responses detected in both assays were related to the viral load during infection as assessed by the CMV antigenemia test, which is a quantitative marker for CMV load. It was found that although epitopes on naturally occurring proteins induce higher antibody responses and responses in more patients, antibodies directed to epitopes present on the recombinant proteins were inversely related to the viral load during a CMV infection. Therefore, antibodies to epitopes on the recombinant proteins might be more clinically relevant in this group of lung transplant recipients.

Authors+Show Affiliations

Department of Clinical Immunology, University Hospital Groningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7535179

Citation

van Zanten, J, et al. "Humoral Immune Response Against Human Cytomegalovirus (HCMV)-specific Proteins After HCMV Infection in Lung Transplantation as Detected With Recombinant and Naturally Occurring Proteins." Clinical and Diagnostic Laboratory Immunology, vol. 2, no. 2, 1995, pp. 214-8.
van Zanten J, Harmsen MC, van der Giessen M, et al. Humoral immune response against human cytomegalovirus (HCMV)-specific proteins after HCMV infection in lung transplantation as detected with recombinant and naturally occurring proteins. Clin Diagn Lab Immunol. 1995;2(2):214-8.
van Zanten, J., Harmsen, M. C., van der Giessen, M., van der Bij, W., Prop, J., de Leij, L., & The, T. H. (1995). Humoral immune response against human cytomegalovirus (HCMV)-specific proteins after HCMV infection in lung transplantation as detected with recombinant and naturally occurring proteins. Clinical and Diagnostic Laboratory Immunology, 2(2), 214-8.
van Zanten J, et al. Humoral Immune Response Against Human Cytomegalovirus (HCMV)-specific Proteins After HCMV Infection in Lung Transplantation as Detected With Recombinant and Naturally Occurring Proteins. Clin Diagn Lab Immunol. 1995;2(2):214-8. PubMed PMID: 7535179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Humoral immune response against human cytomegalovirus (HCMV)-specific proteins after HCMV infection in lung transplantation as detected with recombinant and naturally occurring proteins. AU - van Zanten,J, AU - Harmsen,M C, AU - van der Giessen,M, AU - van der Bij,W, AU - Prop,J, AU - de Leij,L, AU - The,T H, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 214 EP - 8 JF - Clinical and diagnostic laboratory immunology JO - Clin Diagn Lab Immunol VL - 2 IS - 2 N2 - The humoral immune response to four intracellularly located cytomegalovirus (CMV) proteins was studied in 15 lung transplant recipients experiencing active CMV infections. Five patients had primary infections, and 10 had secondary infections. Antibodies of the immunoglobulin M (IgM) and IgG classes were measured in an enzyme-linked immunosorbent assay (ELISA) system in which procaryotically expressed recombinant proteins were used as a substrate and also in a monoclonal antibody-based capture ELISA which uses naturally occurring proteins as a substrate. The proteins investigated were the lower matrix protein pp65 (ppUL83), the major DNA-binding protein p52 (ppUL44), and the two immediate early proteins IE1 and IE2 (different splicing products of UL123). Higher levels of antibodies were found to pp65 and especially to p52 than to the immediate early antigens. Antibody levels detected in the recombinant protein-based ELISAs were generally lower than antibody responses detected with the matching antigen capture ELISA. Moreover, some patients appeared to have antibodies mainly to epitopes present on naturally occurring proteins. The antibody responses detected in both assays were related to the viral load during infection as assessed by the CMV antigenemia test, which is a quantitative marker for CMV load. It was found that although epitopes on naturally occurring proteins induce higher antibody responses and responses in more patients, antibodies directed to epitopes present on the recombinant proteins were inversely related to the viral load during a CMV infection. Therefore, antibodies to epitopes on the recombinant proteins might be more clinically relevant in this group of lung transplant recipients. SN - 1071-412X UR - https://www.unboundmedicine.com/medline/citation/7535179/Humoral_immune_response_against_human_cytomegalovirus__HCMV__specific_proteins_after_HCMV_infection_in_lung_transplantation_as_detected_with_recombinant_and_naturally_occurring_proteins_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=7535179 DB - PRIME DP - Unbound Medicine ER -