Tags

Type your tag names separated by a space and hit enter

Autoimmune antiphospholipid antibodies are directed against a cryptic epitope expressed when beta 2-glycoprotein I is bound to a suitable surface.
Thromb Haemost. 1995 Jan; 73(1):29-34.TH

Abstract

The antiphospholipid antibodies (aPL) present in autoimmune disorders are associated with thromboembolic episodes, and their binding to phospholipids (PL) is mediated by a plasma cofactor, beta 2-glycoprotein I (beta 2GPI). Both PL and beta 2GPI seem necessary for binding, thus indicating that the two components comprise the epitope against which aPL are directed. Using an anti-beta 2GPI antibody ELISA with the antigen adsorbed onto polyvinylchloride (PVC) plates, we detected high antibody titres in 12 out of 12 plasma from patients with the antiphospholipid syndrome. No or very low positivity was obtained when the same ELISA was carried out in polystyrene (PST) plates, while an increasing positivity was found when processed (i.e. more hydrophilic) or COOH-surface PST plates were used. When beta 2GPI dependent IgG-aPL were purified using agarose-immobilized cardiolipin, 4 out of 4 preparations were highly positive in anti-beta 2GPI antibody ELISA using PVC plates, while beta 2GPI was not fully recognized by aPL-IgG when adsorbed onto PST plates. These findings demonstrate that aPL are, in fact, anti-beta 2GPI antibodies directed against a cryptic epitope which is expressed when beta 2GPI is bound to anionic phospholipid, or another suitable surface.

Authors+Show Affiliations

Dept. of Cardiology, University of Padua School of Medicine, Italy.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7537919

Citation

Pengo, V, et al. "Autoimmune Antiphospholipid Antibodies Are Directed Against a Cryptic Epitope Expressed when Beta 2-glycoprotein I Is Bound to a Suitable Surface." Thrombosis and Haemostasis, vol. 73, no. 1, 1995, pp. 29-34.
Pengo V, Biasiolo A, Fior MG. Autoimmune antiphospholipid antibodies are directed against a cryptic epitope expressed when beta 2-glycoprotein I is bound to a suitable surface. Thromb Haemost. 1995;73(1):29-34.
Pengo, V., Biasiolo, A., & Fior, M. G. (1995). Autoimmune antiphospholipid antibodies are directed against a cryptic epitope expressed when beta 2-glycoprotein I is bound to a suitable surface. Thrombosis and Haemostasis, 73(1), 29-34.
Pengo V, Biasiolo A, Fior MG. Autoimmune Antiphospholipid Antibodies Are Directed Against a Cryptic Epitope Expressed when Beta 2-glycoprotein I Is Bound to a Suitable Surface. Thromb Haemost. 1995;73(1):29-34. PubMed PMID: 7537919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoimmune antiphospholipid antibodies are directed against a cryptic epitope expressed when beta 2-glycoprotein I is bound to a suitable surface. AU - Pengo,V, AU - Biasiolo,A, AU - Fior,M G, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 29 EP - 34 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 73 IS - 1 N2 - The antiphospholipid antibodies (aPL) present in autoimmune disorders are associated with thromboembolic episodes, and their binding to phospholipids (PL) is mediated by a plasma cofactor, beta 2-glycoprotein I (beta 2GPI). Both PL and beta 2GPI seem necessary for binding, thus indicating that the two components comprise the epitope against which aPL are directed. Using an anti-beta 2GPI antibody ELISA with the antigen adsorbed onto polyvinylchloride (PVC) plates, we detected high antibody titres in 12 out of 12 plasma from patients with the antiphospholipid syndrome. No or very low positivity was obtained when the same ELISA was carried out in polystyrene (PST) plates, while an increasing positivity was found when processed (i.e. more hydrophilic) or COOH-surface PST plates were used. When beta 2GPI dependent IgG-aPL were purified using agarose-immobilized cardiolipin, 4 out of 4 preparations were highly positive in anti-beta 2GPI antibody ELISA using PVC plates, while beta 2GPI was not fully recognized by aPL-IgG when adsorbed onto PST plates. These findings demonstrate that aPL are, in fact, anti-beta 2GPI antibodies directed against a cryptic epitope which is expressed when beta 2GPI is bound to anionic phospholipid, or another suitable surface. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/7537919/Autoimmune_antiphospholipid_antibodies_are_directed_against_a_cryptic_epitope_expressed_when_beta_2_glycoprotein_I_is_bound_to_a_suitable_surface_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:7537919 DB - PRIME DP - Unbound Medicine ER -