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Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species.
Appl Environ Microbiol 1995; 61(4):1623-6AE

Abstract

Bacillus thuringiensis spacer regions between the 16S and 23S rRNAs were amplified with conserved primers, designated 19-mer and 23-mer primers. A spacer region of 144 bp was determined for all of 6 B. thuringiensis strains, 7 B. thuringiensis subspecies, and 11 B. thuringiensis field isolates, as well as for the closely related species Bacillus cereus and Bacillus anthracis. Computer analysis and alignment of nucleotide sequences identified three mutations and one deletion in the intergenic spacer region (ISR) of B. thuringiensis subsp. kurstaki HD-1 when compared with ISR sequences from other subspecies. The same differences were identified between the ISR of B. thuringiensis strains and the ISR of B. cereus and B. anthracis. These minor differences do not seem to be sufficient to allow the design of a species-specific oligonucleotide probe.

Authors+Show Affiliations

Natural Resources Canada, Canadian Forest Service--Quebec Region, Sainte-Foy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7538281

Citation

Bourque, S N., et al. "Comparative Analysis of the 16S to 23S Ribosomal Intergenic Spacer Sequences of Bacillus Thuringiensis Strains and Subspecies and of Closely Related Species." Applied and Environmental Microbiology, vol. 61, no. 4, 1995, pp. 1623-6.
Bourque SN, Valero JR, Lavoie MC, et al. Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species. Appl Environ Microbiol. 1995;61(4):1623-6.
Bourque, S. N., Valero, J. R., Lavoie, M. C., & Levesque, R. C. (1995). Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species. Applied and Environmental Microbiology, 61(4), pp. 1623-6.
Bourque SN, et al. Comparative Analysis of the 16S to 23S Ribosomal Intergenic Spacer Sequences of Bacillus Thuringiensis Strains and Subspecies and of Closely Related Species. Appl Environ Microbiol. 1995;61(4):1623-6. PubMed PMID: 7538281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species. AU - Bourque,S N, AU - Valero,J R, AU - Lavoie,M C, AU - Levesque,R C, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 1623 EP - 6 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 61 IS - 4 N2 - Bacillus thuringiensis spacer regions between the 16S and 23S rRNAs were amplified with conserved primers, designated 19-mer and 23-mer primers. A spacer region of 144 bp was determined for all of 6 B. thuringiensis strains, 7 B. thuringiensis subspecies, and 11 B. thuringiensis field isolates, as well as for the closely related species Bacillus cereus and Bacillus anthracis. Computer analysis and alignment of nucleotide sequences identified three mutations and one deletion in the intergenic spacer region (ISR) of B. thuringiensis subsp. kurstaki HD-1 when compared with ISR sequences from other subspecies. The same differences were identified between the ISR of B. thuringiensis strains and the ISR of B. cereus and B. anthracis. These minor differences do not seem to be sufficient to allow the design of a species-specific oligonucleotide probe. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/7538281/Comparative_analysis_of_the_16S_to_23S_ribosomal_intergenic_spacer_sequences_of_Bacillus_thuringiensis_strains_and_subspecies_and_of_closely_related_species_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=7538281 DB - PRIME DP - Unbound Medicine ER -