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Influence of TGF-beta on murine thymocyte development in fetal thymus organ culture.
J Immunol. 1995 Jun 01; 154(11):5789-98.JI

Abstract

TGF-beta is a multifunctional growth regulator that can either inhibit or stimulate the growth and differentiation of lymphocytes. For several cell types the effect of TGF-beta was found to correlate with the differentiation stage of the cells. We have studied the influence of TGF-beta on the differentiation of murine thymocytes by evaluating the effect of TGF-beta on the generation of thymocyte subpopulations in fetal thymus organ culture. TGF-beta inhibited the growth and differentiation of CD4-CD8- double-negative thymocytes. In the CD4-CD8- double-negative cell population, most cells remained CD44+CD25-, with CD44+CD25+ and CD44-CD25- subpopulations dramatically decreased in cell numbers. The accumulation of cells with a phenotype characteristic of cells in early stage of differentiation suggests a block at very early transition steps. These observations were confirmed in experiments with precursor cells from fetal liver transferred to 2-deoxyguanosine-treated alymphoid thymic lobes, inasmuch as addition of TGF-beta caused a complete inhibition of T cell development. Differentiation into CD4+CD8+ double-positive thymocytes and CD4+ single-positive thymocytes was impaired because these cell numbers were greatly reduced. In contrast, the CD8+ single-positive subpopulation retained normal cell numbers. This CD8+ population had characteristics of a mature subset as the cells expressed CD8 beta and high levels of TCR-alpha beta and CD3. This TCR-alpha beta + cell population was not actively dividing, suggesting that these cells arise de novo by differentiation.

Authors+Show Affiliations

Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7538530

Citation

Plum, J, et al. "Influence of TGF-beta On Murine Thymocyte Development in Fetal Thymus Organ Culture." Journal of Immunology (Baltimore, Md. : 1950), vol. 154, no. 11, 1995, pp. 5789-98.
Plum J, De Smedt M, Leclercq G, et al. Influence of TGF-beta on murine thymocyte development in fetal thymus organ culture. J Immunol. 1995;154(11):5789-98.
Plum, J., De Smedt, M., Leclercq, G., & Vandekerckhove, B. (1995). Influence of TGF-beta on murine thymocyte development in fetal thymus organ culture. Journal of Immunology (Baltimore, Md. : 1950), 154(11), 5789-98.
Plum J, et al. Influence of TGF-beta On Murine Thymocyte Development in Fetal Thymus Organ Culture. J Immunol. 1995 Jun 1;154(11):5789-98. PubMed PMID: 7538530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of TGF-beta on murine thymocyte development in fetal thymus organ culture. AU - Plum,J, AU - De Smedt,M, AU - Leclercq,G, AU - Vandekerckhove,B, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 5789 EP - 98 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 154 IS - 11 N2 - TGF-beta is a multifunctional growth regulator that can either inhibit or stimulate the growth and differentiation of lymphocytes. For several cell types the effect of TGF-beta was found to correlate with the differentiation stage of the cells. We have studied the influence of TGF-beta on the differentiation of murine thymocytes by evaluating the effect of TGF-beta on the generation of thymocyte subpopulations in fetal thymus organ culture. TGF-beta inhibited the growth and differentiation of CD4-CD8- double-negative thymocytes. In the CD4-CD8- double-negative cell population, most cells remained CD44+CD25-, with CD44+CD25+ and CD44-CD25- subpopulations dramatically decreased in cell numbers. The accumulation of cells with a phenotype characteristic of cells in early stage of differentiation suggests a block at very early transition steps. These observations were confirmed in experiments with precursor cells from fetal liver transferred to 2-deoxyguanosine-treated alymphoid thymic lobes, inasmuch as addition of TGF-beta caused a complete inhibition of T cell development. Differentiation into CD4+CD8+ double-positive thymocytes and CD4+ single-positive thymocytes was impaired because these cell numbers were greatly reduced. In contrast, the CD8+ single-positive subpopulation retained normal cell numbers. This CD8+ population had characteristics of a mature subset as the cells expressed CD8 beta and high levels of TCR-alpha beta and CD3. This TCR-alpha beta + cell population was not actively dividing, suggesting that these cells arise de novo by differentiation. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7538530/Influence_of_TGF_beta_on_murine_thymocyte_development_in_fetal_thymus_organ_culture_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7538530 DB - PRIME DP - Unbound Medicine ER -