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[Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells].
Yao Xue Xue Bao. 1995; 30(3):235-8.YX

Abstract

4-Aminopyridine (4-AP) has been shown to induce histamine release from isolated peritoneal mast cells (PMC) in mice and rats. In the presence of extracellular calcium at normal level (0.9 mmol.L-1) histamine release induced by 4-AP (13.6 mmol.L-1) from mice PMC was 33.0% +/- 4.6%, while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 25.5% +/- 4.2% and 16.3% +/- 3.7% respectively. Histamine release in response to 4-AP (10 mmol.L-1) from rat PMC was 39.1% +/- 6.7% (0.9 mmol.L-1 calcium), while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 29.3% +/- 4.7% and 20.2% +/- 2.9% respectively. Results of statistical analysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMC were preincubated in calcium-free medium with EDTA 0.1 mmol.L-1 for 180 min 4-AP induced histamine release was 13.8% +/- 1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells. The mechanism of 4-AP induced histamine release was discussed.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Zhejiang Medical University, Hangzhou.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

7543724

Citation

Xu, J H., et al. "[Influence of Calcium Concentration On 4-aminopyridine Induced Histamine Release From Isolated Peritoneal Mast Cells]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 30, no. 3, 1995, pp. 235-8.
Xu JH, Wang P, Chen ZG, et al. [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. Yao Xue Xue Bao. 1995;30(3):235-8.
Xu, J. H., Wang, P., Chen, Z. G., & Zhang, J. X. (1995). [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 30(3), 235-8.
Xu JH, et al. [Influence of Calcium Concentration On 4-aminopyridine Induced Histamine Release From Isolated Peritoneal Mast Cells]. Yao Xue Xue Bao. 1995;30(3):235-8. PubMed PMID: 7543724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. AU - Xu,J H, AU - Wang,P, AU - Chen,Z G, AU - Zhang,J X, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 235 EP - 8 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 30 IS - 3 N2 - 4-Aminopyridine (4-AP) has been shown to induce histamine release from isolated peritoneal mast cells (PMC) in mice and rats. In the presence of extracellular calcium at normal level (0.9 mmol.L-1) histamine release induced by 4-AP (13.6 mmol.L-1) from mice PMC was 33.0% +/- 4.6%, while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 25.5% +/- 4.2% and 16.3% +/- 3.7% respectively. Histamine release in response to 4-AP (10 mmol.L-1) from rat PMC was 39.1% +/- 6.7% (0.9 mmol.L-1 calcium), while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 29.3% +/- 4.7% and 20.2% +/- 2.9% respectively. Results of statistical analysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMC were preincubated in calcium-free medium with EDTA 0.1 mmol.L-1 for 180 min 4-AP induced histamine release was 13.8% +/- 1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells. The mechanism of 4-AP induced histamine release was discussed. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/7543724/[Influence_of_calcium_concentration_on_4_aminopyridine_induced_histamine_release_from_isolated_peritoneal_mast_cells]_ DB - PRIME DP - Unbound Medicine ER -