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[Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells].
Yao Xue Xue Bao. 1995; 30(3):235-8.YX

Abstract

4-Aminopyridine (4-AP) has been shown to induce histamine release from isolated peritoneal mast cells (PMC) in mice and rats. In the presence of extracellular calcium at normal level (0.9 mmol.L-1) histamine release induced by 4-AP (13.6 mmol.L-1) from mice PMC was 33.0% +/- 4.6%, while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 25.5% +/- 4.2% and 16.3% +/- 3.7% respectively. Histamine release in response to 4-AP (10 mmol.L-1) from rat PMC was 39.1% +/- 6.7% (0.9 mmol.L-1 calcium), while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 29.3% +/- 4.7% and 20.2% +/- 2.9% respectively. Results of statistical analysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMC were preincubated in calcium-free medium with EDTA 0.1 mmol.L-1 for 180 min 4-AP induced histamine release was 13.8% +/- 1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells. The mechanism of 4-AP induced histamine release was discussed.

Authors+Show Affiliations

Xu JH
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Zhejiang Medical University, Hangzhou.
Wang P
No affiliation info available
Chen ZG
No affiliation info available
Zhang JX
No affiliation info available

MeSH

4-AminopyridineAnimalsBiological Transport, ActiveCalciumFemaleHistamine ReleaseMaleMast CellsMicePeritoneal CavityRatsRats, Sprague-Dawley

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

7543724

Citation

Xu, J H., et al. "[Influence of Calcium Concentration On 4-aminopyridine Induced Histamine Release From Isolated Peritoneal Mast Cells]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 30, no. 3, 1995, pp. 235-8.
Xu JH, Wang P, Chen ZG, et al. [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. Yao Xue Xue Bao. 1995;30(3):235-8.
Xu, J. H., Wang, P., Chen, Z. G., & Zhang, J. X. (1995). [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 30(3), 235-8.
Xu JH, et al. [Influence of Calcium Concentration On 4-aminopyridine Induced Histamine Release From Isolated Peritoneal Mast Cells]. Yao Xue Xue Bao. 1995;30(3):235-8. PubMed PMID: 7543724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells]. AU - Xu,J H, AU - Wang,P, AU - Chen,Z G, AU - Zhang,J X, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 235 EP - 8 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 30 IS - 3 N2 - 4-Aminopyridine (4-AP) has been shown to induce histamine release from isolated peritoneal mast cells (PMC) in mice and rats. In the presence of extracellular calcium at normal level (0.9 mmol.L-1) histamine release induced by 4-AP (13.6 mmol.L-1) from mice PMC was 33.0% +/- 4.6%, while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 25.5% +/- 4.2% and 16.3% +/- 3.7% respectively. Histamine release in response to 4-AP (10 mmol.L-1) from rat PMC was 39.1% +/- 6.7% (0.9 mmol.L-1 calcium), while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 29.3% +/- 4.7% and 20.2% +/- 2.9% respectively. Results of statistical analysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMC were preincubated in calcium-free medium with EDTA 0.1 mmol.L-1 for 180 min 4-AP induced histamine release was 13.8% +/- 1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells. The mechanism of 4-AP induced histamine release was discussed. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/7543724/[Influence_of_calcium_concentration_on_4_aminopyridine_induced_histamine_release_from_isolated_peritoneal_mast_cells]_ DB - PRIME DP - Unbound Medicine ER -