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Role of thromboxane A2 in bradykinin-induced human isolated small bronchi contraction.
Eur J Pharmacol. 1995 May 04; 278(1):49-54.EJ

Abstract

We previously demonstrated that the bradykinin-induced contraction of human isolated small bronchi is inhibited by indomethacin, capsaicin (N-methyl-N-6-nonenamide) and ruthenium red but not by tachykinin receptor antagonists. The thromboxane A2 receptor (TP receptor) antagonist GR32191 ((1R-(1 alpha(Z),2 beta,3 beta,5 alpha))-(+)-7-(5-(((1,1'-biphenyl)-4-yl)- methoxy)-3-hydroxy-2-(1-piperidinyl)cyclopentyl)-4-heptenoic acid, hydrochloride) (10(-10) to 10(-8) M) dose dependently inhibited the effect of bradykinin, suggesting the mediation of the TP receptor in the action of bradykinin. With higher concentrations of GR32191 (10(-7) and 10(-6) M) bradykinin induced a relaxation which was inhibited by indomethacin and by the bradykinin B2 receptor antagonist Hoe 140 (D-Arg0[Hyp3,Thi-5,D-Tic7,Oic8]bradykinin). The thromboxane A2 synthase inhibitor dazoxiben (4-(-2-(1H-imidazol-1-yl)ethoxy) benzoic acid hydrochloride) 10(-6) M inhibited the bradykinin-induced contraction, suggesting that thromboxane A2 was involved in TP receptor stimulation. The thromboxane A2 mimetic U-46619 (9,11-dideoxy-11 alpha,9 alpha-epoxy-methano-prostaglandin F2 alpha)-induced contraction of human distal bronchi was not inhibited by capsaicin and ruthenium red. Our data suggest that bradykinin contracts human isolated small bronchi through thromboxane A2 release. The inhibitory effect of ruthenium red and capsaicin on the bradykinin response may be due to inhibition of thromboxane A2 release or arachidonic mobilisation.

Authors+Show Affiliations

Laboratoire de Pharmacologie, Faculté de Médecine Paris Ouest, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7545124

Citation

Molimard, M, et al. "Role of Thromboxane A2 in Bradykinin-induced Human Isolated Small Bronchi Contraction." European Journal of Pharmacology, vol. 278, no. 1, 1995, pp. 49-54.
Molimard M, Martin CA, Naline E, et al. Role of thromboxane A2 in bradykinin-induced human isolated small bronchi contraction. Eur J Pharmacol. 1995;278(1):49-54.
Molimard, M., Martin, C. A., Naline, E., Hirsch, A., & Advenier, C. (1995). Role of thromboxane A2 in bradykinin-induced human isolated small bronchi contraction. European Journal of Pharmacology, 278(1), 49-54.
Molimard M, et al. Role of Thromboxane A2 in Bradykinin-induced Human Isolated Small Bronchi Contraction. Eur J Pharmacol. 1995 May 4;278(1):49-54. PubMed PMID: 7545124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of thromboxane A2 in bradykinin-induced human isolated small bronchi contraction. AU - Molimard,M, AU - Martin,C A, AU - Naline,E, AU - Hirsch,A, AU - Advenier,C, PY - 1995/5/4/pubmed PY - 1995/5/4/medline PY - 1995/5/4/entrez SP - 49 EP - 54 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 278 IS - 1 N2 - We previously demonstrated that the bradykinin-induced contraction of human isolated small bronchi is inhibited by indomethacin, capsaicin (N-methyl-N-6-nonenamide) and ruthenium red but not by tachykinin receptor antagonists. The thromboxane A2 receptor (TP receptor) antagonist GR32191 ((1R-(1 alpha(Z),2 beta,3 beta,5 alpha))-(+)-7-(5-(((1,1'-biphenyl)-4-yl)- methoxy)-3-hydroxy-2-(1-piperidinyl)cyclopentyl)-4-heptenoic acid, hydrochloride) (10(-10) to 10(-8) M) dose dependently inhibited the effect of bradykinin, suggesting the mediation of the TP receptor in the action of bradykinin. With higher concentrations of GR32191 (10(-7) and 10(-6) M) bradykinin induced a relaxation which was inhibited by indomethacin and by the bradykinin B2 receptor antagonist Hoe 140 (D-Arg0[Hyp3,Thi-5,D-Tic7,Oic8]bradykinin). The thromboxane A2 synthase inhibitor dazoxiben (4-(-2-(1H-imidazol-1-yl)ethoxy) benzoic acid hydrochloride) 10(-6) M inhibited the bradykinin-induced contraction, suggesting that thromboxane A2 was involved in TP receptor stimulation. The thromboxane A2 mimetic U-46619 (9,11-dideoxy-11 alpha,9 alpha-epoxy-methano-prostaglandin F2 alpha)-induced contraction of human distal bronchi was not inhibited by capsaicin and ruthenium red. Our data suggest that bradykinin contracts human isolated small bronchi through thromboxane A2 release. The inhibitory effect of ruthenium red and capsaicin on the bradykinin response may be due to inhibition of thromboxane A2 release or arachidonic mobilisation. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/7545124/Role_of_thromboxane_A2_in_bradykinin_induced_human_isolated_small_bronchi_contraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/001429999500103R DB - PRIME DP - Unbound Medicine ER -