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Determination of free concentration of ropivacaine and bupivacaine in blood plasma by ultrafiltration and coupled-column liquid chromatography.
J Chromatogr B Biomed Appl. 1995 Jun 09; 668(1):91-8.JC

Abstract

A coupled-column liquid chromatographic method for determining the free concentration of ropivacaine and bupivacaine in blood plasma was developed. Following adjustment of the temperature and pH, the plasma samples were ultrafiltrated. Ropivacaine or bupivacaine in the ultrafiltrate was determined by direct injection into a coupled-column liquid chromatographic system, consisting of one reversed-phase and one ion-exchange column. The system was highly selective. Ropivacaine and bupivacaine were detected by UV at 210 nm. The limit of determination was 10 nM and the inter-assay precision at a concentration level of about 100 nM was 6% (R.S.D., n = 30) for ropivacaine and 7% (R.S.D., n = 30) for bupivacaine.

Authors+Show Affiliations

Astra Pain Control AB, Södertälje, Sweden.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7550986

Citation

Arvidsson, T, and E Eklund. "Determination of Free Concentration of Ropivacaine and Bupivacaine in Blood Plasma By Ultrafiltration and Coupled-column Liquid Chromatography." Journal of Chromatography. B, Biomedical Applications, vol. 668, no. 1, 1995, pp. 91-8.
Arvidsson T, Eklund E. Determination of free concentration of ropivacaine and bupivacaine in blood plasma by ultrafiltration and coupled-column liquid chromatography. J Chromatogr B Biomed Appl. 1995;668(1):91-8.
Arvidsson, T., & Eklund, E. (1995). Determination of free concentration of ropivacaine and bupivacaine in blood plasma by ultrafiltration and coupled-column liquid chromatography. Journal of Chromatography. B, Biomedical Applications, 668(1), 91-8.
Arvidsson T, Eklund E. Determination of Free Concentration of Ropivacaine and Bupivacaine in Blood Plasma By Ultrafiltration and Coupled-column Liquid Chromatography. J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):91-8. PubMed PMID: 7550986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of free concentration of ropivacaine and bupivacaine in blood plasma by ultrafiltration and coupled-column liquid chromatography. AU - Arvidsson,T, AU - Eklund,E, PY - 1995/6/9/pubmed PY - 1995/6/9/medline PY - 1995/6/9/entrez SP - 91 EP - 8 JF - Journal of chromatography. B, Biomedical applications JO - J Chromatogr B Biomed Appl VL - 668 IS - 1 N2 - A coupled-column liquid chromatographic method for determining the free concentration of ropivacaine and bupivacaine in blood plasma was developed. Following adjustment of the temperature and pH, the plasma samples were ultrafiltrated. Ropivacaine or bupivacaine in the ultrafiltrate was determined by direct injection into a coupled-column liquid chromatographic system, consisting of one reversed-phase and one ion-exchange column. The system was highly selective. Ropivacaine and bupivacaine were detected by UV at 210 nm. The limit of determination was 10 nM and the inter-assay precision at a concentration level of about 100 nM was 6% (R.S.D., n = 30) for ropivacaine and 7% (R.S.D., n = 30) for bupivacaine. SN - 1572-6495 UR - https://www.unboundmedicine.com/medline/citation/7550986/Determination_of_free_concentration_of_ropivacaine_and_bupivacaine_in_blood_plasma_by_ultrafiltration_and_coupled_column_liquid_chromatography_ L2 - https://ClinicalTrials.gov/search/term=7550986 [PUBMED-IDS] DB - PRIME DP - Unbound Medicine ER -