Tags

Type your tag names separated by a space and hit enter

Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction.
J Clin Microbiol 1995; 33(8):2042-7JC

Abstract

The suitability of urine specimens from women and men for the detection of Chlamydia trachomatis infection by a ligase chain reaction (LCR)-based assay with plasmid primers was examined with a group of patients attending a sexually transmitted disease clinic in Amsterdam, The Netherlands. Cervical specimens from 15 of 237 (6.3%) women tested positive for C. trachomatis by cell culture. Of the 25 (10.5%) female urine samples that tested positive by the plasmid-LCR assay, 13 were obtained from cervical culture-positive women. Nine of the 12 plasmid-LCR-positive urine samples from cervical culture-negative women were confirmed to be positive by a second LCR assay with primers based on chromosomal DNA. Urethral specimens from 24 of 258 (9.3%) men were positive for C. trachomatis infection by cell culture. Of the 25 (9.7%) urine samples that tested positive by plasmid-LCR, 20 were from culture-positive men. All five of the LCR-positive urine samples from culture-negative men were confirmed to be positive by the LCR with chromosomal DNA primers. Relative to cell culture, testing by plasmid-LCR analysis of male urine samples had a sensitivity of 83.3% and a specificity of 97.9%; after resolution of discordant samples, these values were 86.2 and 100%, respectively. In the study with women, the sensitivities of plasmid-LCR analysis of cervical and urine specimens in comparison with cervical cell culture were 93.3 and 86.7%, respectively. After resolution of discrepant samples, the sensitivities of the plasmid-LCR test for cervical swabs and female urine samples were 96.3 and 92.6%, respectively. These results indicate that the plasmid-LCR-based assay is a very reliable, sensitive, convenient test for the detection of C. trachomatis infection in female and male urine specimens.

Authors+Show Affiliations

Department of Public Health, Municipal Health Service of Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7559945

Citation

van Doornum, G J., et al. "Detection of Chlamydia Trachomatis Infection in Urine Samples From Men and Women By Ligase Chain Reaction." Journal of Clinical Microbiology, vol. 33, no. 8, 1995, pp. 2042-7.
van Doornum GJ, Buimer M, Prins M, et al. Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction. J Clin Microbiol. 1995;33(8):2042-7.
van Doornum, G. J., Buimer, M., Prins, M., Henquet, C. J., Coutinho, R. A., Plier, P. K., ... Lee, H. (1995). Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction. Journal of Clinical Microbiology, 33(8), pp. 2042-7.
van Doornum GJ, et al. Detection of Chlamydia Trachomatis Infection in Urine Samples From Men and Women By Ligase Chain Reaction. J Clin Microbiol. 1995;33(8):2042-7. PubMed PMID: 7559945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction. AU - van Doornum,G J, AU - Buimer,M, AU - Prins,M, AU - Henquet,C J, AU - Coutinho,R A, AU - Plier,P K, AU - Tomazic-Allen,S, AU - Hu,H, AU - Lee,H, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 2042 EP - 7 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 33 IS - 8 N2 - The suitability of urine specimens from women and men for the detection of Chlamydia trachomatis infection by a ligase chain reaction (LCR)-based assay with plasmid primers was examined with a group of patients attending a sexually transmitted disease clinic in Amsterdam, The Netherlands. Cervical specimens from 15 of 237 (6.3%) women tested positive for C. trachomatis by cell culture. Of the 25 (10.5%) female urine samples that tested positive by the plasmid-LCR assay, 13 were obtained from cervical culture-positive women. Nine of the 12 plasmid-LCR-positive urine samples from cervical culture-negative women were confirmed to be positive by a second LCR assay with primers based on chromosomal DNA. Urethral specimens from 24 of 258 (9.3%) men were positive for C. trachomatis infection by cell culture. Of the 25 (9.7%) urine samples that tested positive by plasmid-LCR, 20 were from culture-positive men. All five of the LCR-positive urine samples from culture-negative men were confirmed to be positive by the LCR with chromosomal DNA primers. Relative to cell culture, testing by plasmid-LCR analysis of male urine samples had a sensitivity of 83.3% and a specificity of 97.9%; after resolution of discordant samples, these values were 86.2 and 100%, respectively. In the study with women, the sensitivities of plasmid-LCR analysis of cervical and urine specimens in comparison with cervical cell culture were 93.3 and 86.7%, respectively. After resolution of discrepant samples, the sensitivities of the plasmid-LCR test for cervical swabs and female urine samples were 96.3 and 92.6%, respectively. These results indicate that the plasmid-LCR-based assay is a very reliable, sensitive, convenient test for the detection of C. trachomatis infection in female and male urine specimens. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7559945/Detection_of_Chlamydia_trachomatis_infection_in_urine_samples_from_men_and_women_by_ligase_chain_reaction_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=7559945 DB - PRIME DP - Unbound Medicine ER -