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Regulation of the transcription factor C/EBP alpha following peritoneal sepsis.
J Surg Res. 1995 Oct; 59(4):460-7.JS

Abstract

The transcription factors C/EBP alpha and C/EBP beta belong to the leucine-zipper C/EBP (CCAAT/enhancer binding protein) family of DNA-binding proteins. C/EBP alpha and C/EBP beta are expressed in the liver and are implicated in the control of transcriptional events following following sepsis. It is hypothesized that inhibition of C/EBP alpha gene expression following sepsis may lead to some of the phenotypic features we recognize as sepsis syndrome such as decreased visceral protein (albumin) synthesis. In this study we demonstrate that C/EBP alpha mRNA accumulation is transiently inhibited 12 hr following peritoneal insult, consistent with previous data. However, we demonstrate that (1) there is increased binding of hepatic nuclear protein to the C/EBP alpha DNA response element 48 hr following insult, (2) a marked increase in C/EBP alpha protein is observed 48 hr following CLP insult compared with no increase in hepatic C/EBP alpha protein at 12 hr postinsult, (3) the increase in hepatic C/EBP alpha protein at 48 hr following cecal ligation and puncture is not associated with an increase in C/EBP alpha mRNA accumulation, (4) the increase in hepatic C/EBP alpha protein is associated with an increase in C/EBP beta protein, and (5) hepatic albumin mRNA accumulation is decreased at 12 and 48 hr following insult and does not correlate with the C/EBP alpha protein synthesis. We conclude that the possible role of the transcription factor C/EBP alpha with respect to decreased albumin gene expression following sepsis must be reevaluated.

Authors+Show Affiliations

Department of Surgery, University of Minnesota, Minneapolis, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7564318

Citation

Chapin, R B., et al. "Regulation of the Transcription Factor C/EBP Alpha Following Peritoneal Sepsis." The Journal of Surgical Research, vol. 59, no. 4, 1995, pp. 460-7.
Chapin RB, Roy S, Charboneau R, et al. Regulation of the transcription factor C/EBP alpha following peritoneal sepsis. J Surg Res. 1995;59(4):460-7.
Chapin, R. B., Roy, S., Charboneau, R., Cain, K., Brady, P. S., Brady, L. J., & Barke, R. A. (1995). Regulation of the transcription factor C/EBP alpha following peritoneal sepsis. The Journal of Surgical Research, 59(4), 460-7.
Chapin RB, et al. Regulation of the Transcription Factor C/EBP Alpha Following Peritoneal Sepsis. J Surg Res. 1995;59(4):460-7. PubMed PMID: 7564318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the transcription factor C/EBP alpha following peritoneal sepsis. AU - Chapin,R B, AU - Roy,S, AU - Charboneau,R, AU - Cain,K, AU - Brady,P S, AU - Brady,L J, AU - Barke,R A, PY - 1995/10/1/pubmed PY - 1995/10/1/medline PY - 1995/10/1/entrez SP - 460 EP - 7 JF - The Journal of surgical research JO - J Surg Res VL - 59 IS - 4 N2 - The transcription factors C/EBP alpha and C/EBP beta belong to the leucine-zipper C/EBP (CCAAT/enhancer binding protein) family of DNA-binding proteins. C/EBP alpha and C/EBP beta are expressed in the liver and are implicated in the control of transcriptional events following following sepsis. It is hypothesized that inhibition of C/EBP alpha gene expression following sepsis may lead to some of the phenotypic features we recognize as sepsis syndrome such as decreased visceral protein (albumin) synthesis. In this study we demonstrate that C/EBP alpha mRNA accumulation is transiently inhibited 12 hr following peritoneal insult, consistent with previous data. However, we demonstrate that (1) there is increased binding of hepatic nuclear protein to the C/EBP alpha DNA response element 48 hr following insult, (2) a marked increase in C/EBP alpha protein is observed 48 hr following CLP insult compared with no increase in hepatic C/EBP alpha protein at 12 hr postinsult, (3) the increase in hepatic C/EBP alpha protein at 48 hr following cecal ligation and puncture is not associated with an increase in C/EBP alpha mRNA accumulation, (4) the increase in hepatic C/EBP alpha protein is associated with an increase in C/EBP beta protein, and (5) hepatic albumin mRNA accumulation is decreased at 12 and 48 hr following insult and does not correlate with the C/EBP alpha protein synthesis. We conclude that the possible role of the transcription factor C/EBP alpha with respect to decreased albumin gene expression following sepsis must be reevaluated. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/7564318/Regulation_of_the_transcription_factor_C/EBP_alpha_following_peritoneal_sepsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(85)71192-4 DB - PRIME DP - Unbound Medicine ER -