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Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver.
J Surg Res. 1995 Oct; 59(4):475-81.JS

Abstract

One of the physiological roles of glutamine is as a precursor for DNA synthesis. The aims of this study were to determine (1) whether glutamine-supplemented total parenteral nutrition (TPN) enhanced DNA and protein synthesis in the liver and (2) whether glutamine uptake was increased following partial hepatectomy in rats. Male Donryu rats (n = 59; body weight, 250-275 g) were randomized into four groups: (1) sham operation + standard TPN solution (C-STPN); (2) C + glutamine-supplemented TPN (C-GTPN); (3) 70% partial hepatectomy + STPN (H-STPN); (4) partial hepatectomy + GTPN (H-GTPN). On Day 0, rats underwent either a sham operation or 70% partial hepatectomy and concomitantly were catheterized in the jugular vein. TPN was begun immediately after the surgery. GTPN was isocaloric and isonitrogenous with STPN and 25% of total nitrogen was given as glutamine. On Day 2, the animals were sacrificed after either a continuous infusion of 1-14C-leucine or a bolus i.v. injection of bromodeoxyuridine. The rate of hepatic regeneration was enhanced with glutamine supplementation (H-STPN, 60.8 +/- 1.6% vs H-GTPN, 66.3 +/- 2.0, P < 0.05) due to an increase in protein synthesis in the liver (H-STPN, 134.0 +/- 10.3%/day vs H-GTPN, 160.9 +/- 6.9, P < 0.05) and DNA synthesis in hepatocytes (H-STPN, 23.1 +/- 2.5% vs H-GTPN, 31.4 +/- 2.9, P < 0.05). The uptake of glutamine by the liver was increased following hepatectomy with GTPN supplementation.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

First Department of Surgery, Kurume University, School of Medicine, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7564320

Citation

Yoshida, S, et al. "Effect of Glutamine Supplement and Hepatectomy On DNA and Protein Synthesis in the Remnant Liver." The Journal of Surgical Research, vol. 59, no. 4, 1995, pp. 475-81.
Yoshida S, Yunoki T, Aoyagi K, et al. Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver. J Surg Res. 1995;59(4):475-81.
Yoshida, S., Yunoki, T., Aoyagi, K., Ohta, J., Ishibashi, N., Noake, T., & Kakegawa, T. (1995). Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver. The Journal of Surgical Research, 59(4), 475-81.
Yoshida S, et al. Effect of Glutamine Supplement and Hepatectomy On DNA and Protein Synthesis in the Remnant Liver. J Surg Res. 1995;59(4):475-81. PubMed PMID: 7564320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver. AU - Yoshida,S, AU - Yunoki,T, AU - Aoyagi,K, AU - Ohta,J, AU - Ishibashi,N, AU - Noake,T, AU - Kakegawa,T, PY - 1995/10/1/pubmed PY - 1995/10/1/medline PY - 1995/10/1/entrez SP - 475 EP - 81 JF - The Journal of surgical research JO - J Surg Res VL - 59 IS - 4 N2 - One of the physiological roles of glutamine is as a precursor for DNA synthesis. The aims of this study were to determine (1) whether glutamine-supplemented total parenteral nutrition (TPN) enhanced DNA and protein synthesis in the liver and (2) whether glutamine uptake was increased following partial hepatectomy in rats. Male Donryu rats (n = 59; body weight, 250-275 g) were randomized into four groups: (1) sham operation + standard TPN solution (C-STPN); (2) C + glutamine-supplemented TPN (C-GTPN); (3) 70% partial hepatectomy + STPN (H-STPN); (4) partial hepatectomy + GTPN (H-GTPN). On Day 0, rats underwent either a sham operation or 70% partial hepatectomy and concomitantly were catheterized in the jugular vein. TPN was begun immediately after the surgery. GTPN was isocaloric and isonitrogenous with STPN and 25% of total nitrogen was given as glutamine. On Day 2, the animals were sacrificed after either a continuous infusion of 1-14C-leucine or a bolus i.v. injection of bromodeoxyuridine. The rate of hepatic regeneration was enhanced with glutamine supplementation (H-STPN, 60.8 +/- 1.6% vs H-GTPN, 66.3 +/- 2.0, P < 0.05) due to an increase in protein synthesis in the liver (H-STPN, 134.0 +/- 10.3%/day vs H-GTPN, 160.9 +/- 6.9, P < 0.05) and DNA synthesis in hepatocytes (H-STPN, 23.1 +/- 2.5% vs H-GTPN, 31.4 +/- 2.9, P < 0.05). The uptake of glutamine by the liver was increased following hepatectomy with GTPN supplementation.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/7564320/Effect_of_glutamine_supplement_and_hepatectomy_on_DNA_and_protein_synthesis_in_the_remnant_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(85)71194-8 DB - PRIME DP - Unbound Medicine ER -