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Characterization of the osmotically inducible gene osmE of Escherichia coli K-12.
Mol Microbiol. 1995 May; 16(3):553-63.MM

Abstract

osmE, an osmotically inducible gene of Escherichia coli, was physically mapped on the bacterial chromosome, cloned and sequenced. osmE appeared to encode a 12,021 Da protein of unknown function, with a lipoprotein-type signal sequence at the amino-terminus. The osmE reading frame was confirmed by sequencing the junction of an osmE-phoA gene fusion. osmE was demonstrated to be transcribed as a single cistron. A phi [osmEp-lac] operon fusion was constructed, and analysis of its expression demonstrated that osmE osmotic regulation probably occurs at the transcriptional level. The osmE promoter was identified by both S1 nuclease and primer extension mapping of the 5' end of the osmE mRNA, by deletion analysis and by identification of a point mutation reducing its activity. Sequence information sufficient for expression and osmotic regulation is present on a DNA fragment extending from positions -37 to +52 with respect to the osmE transcription start. Uninduced expression of the osmE-lac fusion was increased in the presence of mutations in the hns and himA genes. The osmE promoter overlaps a promoter for a gene transcribed in the opposite direction, efg. Transcription from the efg promoter is only weakly affected by osmotic pressure and is independent of the presence of an intact OsmE protein.

Authors+Show Affiliations

Laboratoire de Microbiologie et Génétique Moléculaire, UPR 9007 du CNRS, Toulouse, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7565114

Citation

Gutierrez, C, et al. "Characterization of the Osmotically Inducible Gene osmE of Escherichia Coli K-12." Molecular Microbiology, vol. 16, no. 3, 1995, pp. 553-63.
Gutierrez C, Gordia S, Bonnassie S. Characterization of the osmotically inducible gene osmE of Escherichia coli K-12. Mol Microbiol. 1995;16(3):553-63.
Gutierrez, C., Gordia, S., & Bonnassie, S. (1995). Characterization of the osmotically inducible gene osmE of Escherichia coli K-12. Molecular Microbiology, 16(3), 553-63.
Gutierrez C, Gordia S, Bonnassie S. Characterization of the Osmotically Inducible Gene osmE of Escherichia Coli K-12. Mol Microbiol. 1995;16(3):553-63. PubMed PMID: 7565114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the osmotically inducible gene osmE of Escherichia coli K-12. AU - Gutierrez,C, AU - Gordia,S, AU - Bonnassie,S, PY - 1995/5/1/pubmed PY - 1995/5/1/medline PY - 1995/5/1/entrez SP - 553 EP - 63 JF - Molecular microbiology JO - Mol Microbiol VL - 16 IS - 3 N2 - osmE, an osmotically inducible gene of Escherichia coli, was physically mapped on the bacterial chromosome, cloned and sequenced. osmE appeared to encode a 12,021 Da protein of unknown function, with a lipoprotein-type signal sequence at the amino-terminus. The osmE reading frame was confirmed by sequencing the junction of an osmE-phoA gene fusion. osmE was demonstrated to be transcribed as a single cistron. A phi [osmEp-lac] operon fusion was constructed, and analysis of its expression demonstrated that osmE osmotic regulation probably occurs at the transcriptional level. The osmE promoter was identified by both S1 nuclease and primer extension mapping of the 5' end of the osmE mRNA, by deletion analysis and by identification of a point mutation reducing its activity. Sequence information sufficient for expression and osmotic regulation is present on a DNA fragment extending from positions -37 to +52 with respect to the osmE transcription start. Uninduced expression of the osmE-lac fusion was increased in the presence of mutations in the hns and himA genes. The osmE promoter overlaps a promoter for a gene transcribed in the opposite direction, efg. Transcription from the efg promoter is only weakly affected by osmotic pressure and is independent of the presence of an intact OsmE protein. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/7565114/Characterization_of_the_osmotically_inducible_gene_osmE_of_Escherichia_coli_K_12_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1995&volume=16&issue=3&spage=553 DB - PRIME DP - Unbound Medicine ER -