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Growth factors, tissue and urokinase-type plasminogen activators in venous ulcers.
Ann Cardiol Angeiol (Paris). 1995 Jun-Aug; 44(6):299-303.AC

Abstract

Bioimmunoassays for tissue and urokinase-type plasminogen activators (t-pA and u-pA), and enzyme-linked immunosorbent assays (ELISA) for t-pA were performed on biopsies from the edge and base of 15 venous ulcers. TGF-beta 1, bFGF and PDGF were assessed by ELISA in the edge and base of 19 further venous ulcers and 7 biopsies of normal skin. The presence of all three growth factors and u-pA was confirmed immunohistochemically. T-pA was detected using the ELISA and the bioimmunoassay, but was quantified in 3/15 ulcer bases and 4/15 ulcer edges using the bioimmunoassay only. U-pA was measured in all ulcer samples except one. TGF-beta 1 was measured in 13/19 ulcer bases and 9/19 edges, while free TGF-beta 1 was measured in only 2/19 bases and 4/19 edges. Venous ulcer bases contain significantly greater quantities of u-pA, TGF-beta 1, and bFGF than ulcer edges. TGF-beta 1 was never detected in normal skin. There is significantly less bFGF in normal skin than in venous ulcer bases, but not edges (p = 0.013, p = 0.31 respectively, Mann Whitney U-test). There was a good correlation between ulcer edge TGF-beta 1 and time to healing in ten ulcers that healed within six months from the date of biopsy (r = -0.56, p = 0.065, Spearman Rank Correlation). There was a significantly greater amount of ulcer edge bFGF in the ulcers that healed within six months than those that remained unhealed (p = 0.036, Mann-Whitney U-test).(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Surgery, St Thomas' Hospital, London.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7574362

Citation

Lagattolla, N R., et al. "Growth Factors, Tissue and Urokinase-type Plasminogen Activators in Venous Ulcers." Annales De Cardiologie Et D'angeiologie, vol. 44, no. 6, 1995, pp. 299-303.
Lagattolla NR, Stacey MC, Burnand KG, et al. Growth factors, tissue and urokinase-type plasminogen activators in venous ulcers. Ann Cardiol Angeiol (Paris). 1995;44(6):299-303.
Lagattolla, N. R., Stacey, M. C., Burnand, K. G., & Gaffney, P. G. (1995). Growth factors, tissue and urokinase-type plasminogen activators in venous ulcers. Annales De Cardiologie Et D'angeiologie, 44(6), 299-303.
Lagattolla NR, et al. Growth Factors, Tissue and Urokinase-type Plasminogen Activators in Venous Ulcers. Ann Cardiol Angeiol (Paris). 1995 Jun-Aug;44(6):299-303. PubMed PMID: 7574362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth factors, tissue and urokinase-type plasminogen activators in venous ulcers. AU - Lagattolla,N R, AU - Stacey,M C, AU - Burnand,K G, AU - Gaffney,P G, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 299 EP - 303 JF - Annales de cardiologie et d'angeiologie JO - Ann Cardiol Angeiol (Paris) VL - 44 IS - 6 N2 - Bioimmunoassays for tissue and urokinase-type plasminogen activators (t-pA and u-pA), and enzyme-linked immunosorbent assays (ELISA) for t-pA were performed on biopsies from the edge and base of 15 venous ulcers. TGF-beta 1, bFGF and PDGF were assessed by ELISA in the edge and base of 19 further venous ulcers and 7 biopsies of normal skin. The presence of all three growth factors and u-pA was confirmed immunohistochemically. T-pA was detected using the ELISA and the bioimmunoassay, but was quantified in 3/15 ulcer bases and 4/15 ulcer edges using the bioimmunoassay only. U-pA was measured in all ulcer samples except one. TGF-beta 1 was measured in 13/19 ulcer bases and 9/19 edges, while free TGF-beta 1 was measured in only 2/19 bases and 4/19 edges. Venous ulcer bases contain significantly greater quantities of u-pA, TGF-beta 1, and bFGF than ulcer edges. TGF-beta 1 was never detected in normal skin. There is significantly less bFGF in normal skin than in venous ulcer bases, but not edges (p = 0.013, p = 0.31 respectively, Mann Whitney U-test). There was a good correlation between ulcer edge TGF-beta 1 and time to healing in ten ulcers that healed within six months from the date of biopsy (r = -0.56, p = 0.065, Spearman Rank Correlation). There was a significantly greater amount of ulcer edge bFGF in the ulcers that healed within six months than those that remained unhealed (p = 0.036, Mann-Whitney U-test).(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0003-3928 UR - https://www.unboundmedicine.com/medline/citation/7574362/Growth_factors_tissue_and_urokinase_type_plasminogen_activators_in_venous_ulcers_ L2 - https://antibodies.cancer.gov/detail/CPTC-TGFB1-1 DB - PRIME DP - Unbound Medicine ER -