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High level expression of birch pollen profilin (Bet v 2) in Escherichia coli: purification and characterization of the recombinant allergen.
Biochem Biophys Res Commun. 1995 Oct 04; 215(1):250-63.BB

Abstract

Up to 20% of the population in industrialized countries suffer from type I allergic symptoms (rhinitis, conjunctivitis, and bronchial asthma). The cDNA coding for birch pollen profilin, a highly conserved cross-reactive allergen and actin-binding protein was expressed in Escherichia coli. Upon induction with IPTG up to 30 mg recombinant profilin per liter culture could be obtained. A single step purification protocol based on the high affinity of profilin to poly-(L-proline) Sepharose was used to obtain large amounts of soluble and pure recombinant birch profilin. Recombinant birch pollen profilin specifically bound IgE, elicited dose dependent histamine release from patients basophils and could be used for skin prick testing without toxic effects. The results indicate that by using purified recombinant profilin, specific diagnosis of type I allergy might be improved.

Authors+Show Affiliations

Institute of Molecular Biology, Academy of Science, Salzburg, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7575599

Citation

Susani, M, et al. "High Level Expression of Birch Pollen Profilin (Bet V 2) in Escherichia Coli: Purification and Characterization of the Recombinant Allergen." Biochemical and Biophysical Research Communications, vol. 215, no. 1, 1995, pp. 250-63.
Susani M, Jertschin P, Dolecek C, et al. High level expression of birch pollen profilin (Bet v 2) in Escherichia coli: purification and characterization of the recombinant allergen. Biochem Biophys Res Commun. 1995;215(1):250-63.
Susani, M., Jertschin, P., Dolecek, C., Sperr, W. R., Valent, P., Ebner, C., Kraft, D., Valenta, R., & Scheiner, O. (1995). High level expression of birch pollen profilin (Bet v 2) in Escherichia coli: purification and characterization of the recombinant allergen. Biochemical and Biophysical Research Communications, 215(1), 250-63.
Susani M, et al. High Level Expression of Birch Pollen Profilin (Bet V 2) in Escherichia Coli: Purification and Characterization of the Recombinant Allergen. Biochem Biophys Res Commun. 1995 Oct 4;215(1):250-63. PubMed PMID: 7575599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High level expression of birch pollen profilin (Bet v 2) in Escherichia coli: purification and characterization of the recombinant allergen. AU - Susani,M, AU - Jertschin,P, AU - Dolecek,C, AU - Sperr,W R, AU - Valent,P, AU - Ebner,C, AU - Kraft,D, AU - Valenta,R, AU - Scheiner,O, PY - 1995/10/4/pubmed PY - 1995/10/4/medline PY - 1995/10/4/entrez SP - 250 EP - 63 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 215 IS - 1 N2 - Up to 20% of the population in industrialized countries suffer from type I allergic symptoms (rhinitis, conjunctivitis, and bronchial asthma). The cDNA coding for birch pollen profilin, a highly conserved cross-reactive allergen and actin-binding protein was expressed in Escherichia coli. Upon induction with IPTG up to 30 mg recombinant profilin per liter culture could be obtained. A single step purification protocol based on the high affinity of profilin to poly-(L-proline) Sepharose was used to obtain large amounts of soluble and pure recombinant birch profilin. Recombinant birch pollen profilin specifically bound IgE, elicited dose dependent histamine release from patients basophils and could be used for skin prick testing without toxic effects. The results indicate that by using purified recombinant profilin, specific diagnosis of type I allergy might be improved. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/7575599/High_level_expression_of_birch_pollen_profilin__Bet_v_2__in_Escherichia_coli:_purification_and_characterization_of_the_recombinant_allergen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(85)72460-6 DB - PRIME DP - Unbound Medicine ER -