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IFL- and ELISA-antigliadin antibodies recognize different antigenic reactivities from those of R1-antireticulin and antiendomysial antibodies.
Ital J Gastroenterol. 1995 Mar; 27(2):64-8.IJ

Abstract

In order to establish whether antigliadin antibodies, detected by indirect immunofluorescence (IFL-AGA), display the same antibody reactivity as ELISA-AGA or whether they cross-react with reticulin antibodies (R1-ARA and antiendomysial--EmA), sera from 16 untreated coeliac patients were repeatedly absorbed with crude gliadin dissolved in ethanol/water solution. The comparison between antibody activities before and after absorption demonstrated that IFL-AGA and ELISA-AGA are nothing but a single antibody, as both of them completely disappeared or markedly reduced their titre after incubation with crude gliadin. Moreover, AGA reactivity differs from the reactivity of antibodies directed against reticulin, as R1-ARA and EmA titres were not affected by crude gliadin absorption. The absolute absence of cross-reactivity between IFL-AGA and R1-ARA is further confirmed by the removal of the former and persistence of the latter after gliadin absorption in the 9 sera positive for both antibodies. Sera of coeliac patients, therefore, show only two discrete antibody reactivities, one directed to gliadin and the other to reticulin components.

Authors+Show Affiliations

Medicina Interna 2, Istituto di Clinica Medica Generale e Terapia Medica, Università di Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7579593

Citation

Volta, U, et al. "IFL- and ELISA-antigliadin Antibodies Recognize Different Antigenic Reactivities From Those of R1-antireticulin and Antiendomysial Antibodies." The Italian Journal of Gastroenterology, vol. 27, no. 2, 1995, pp. 64-8.
Volta U, Molinaro N, De Franceschi L, et al. IFL- and ELISA-antigliadin antibodies recognize different antigenic reactivities from those of R1-antireticulin and antiendomysial antibodies. Ital J Gastroenterol. 1995;27(2):64-8.
Volta, U., Molinaro, N., De Franceschi, L., Fratangelo, D., & Bianchi, F. B. (1995). IFL- and ELISA-antigliadin antibodies recognize different antigenic reactivities from those of R1-antireticulin and antiendomysial antibodies. The Italian Journal of Gastroenterology, 27(2), 64-8.
Volta U, et al. IFL- and ELISA-antigliadin Antibodies Recognize Different Antigenic Reactivities From Those of R1-antireticulin and Antiendomysial Antibodies. Ital J Gastroenterol. 1995;27(2):64-8. PubMed PMID: 7579593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IFL- and ELISA-antigliadin antibodies recognize different antigenic reactivities from those of R1-antireticulin and antiendomysial antibodies. AU - Volta,U, AU - Molinaro,N, AU - De Franceschi,L, AU - Fratangelo,D, AU - Bianchi,F B, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 64 EP - 8 JF - The Italian journal of gastroenterology JO - Ital J Gastroenterol VL - 27 IS - 2 N2 - In order to establish whether antigliadin antibodies, detected by indirect immunofluorescence (IFL-AGA), display the same antibody reactivity as ELISA-AGA or whether they cross-react with reticulin antibodies (R1-ARA and antiendomysial--EmA), sera from 16 untreated coeliac patients were repeatedly absorbed with crude gliadin dissolved in ethanol/water solution. The comparison between antibody activities before and after absorption demonstrated that IFL-AGA and ELISA-AGA are nothing but a single antibody, as both of them completely disappeared or markedly reduced their titre after incubation with crude gliadin. Moreover, AGA reactivity differs from the reactivity of antibodies directed against reticulin, as R1-ARA and EmA titres were not affected by crude gliadin absorption. The absolute absence of cross-reactivity between IFL-AGA and R1-ARA is further confirmed by the removal of the former and persistence of the latter after gliadin absorption in the 9 sera positive for both antibodies. Sera of coeliac patients, therefore, show only two discrete antibody reactivities, one directed to gliadin and the other to reticulin components. SN - 0392-0623 UR - https://www.unboundmedicine.com/medline/citation/7579593/IFL__and_ELISA_antigliadin_antibodies_recognize_different_antigenic_reactivities_from_those_of_R1_antireticulin_and_antiendomysial_antibodies_ L2 - https://medlineplus.gov/celiacdisease.html DB - PRIME DP - Unbound Medicine ER -