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Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus.
Braz J Med Biol Res. 1995 Feb; 28(2):183-90.BJ

Abstract

Vicilins (7S storage proteins) from seeds of cowpea (Vigna unguiculata) cultivars which are susceptible or resistant to the bruchid beetle C. maculatus were purified by size-exclusion and ion-exchange chromatography. The vicilins were partially characterized by polyacrylamide gel electrophoresis under both denaturing and nondenaturing conditions, by Western blotting and by amino acid analysis. The variant vicilins from C. maculatus-resistant seeds do not differ appreciably from vicilins from susceptible seeds by these criteria except that they are more strongly bound to DEAE-Sepharose, suggesting differences in charge between the various molecules.

Authors+Show Affiliations

Macedo ML
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Fernandes KV
No affiliation info available
Sales MP
No affiliation info available
Xavier-Filho J
No affiliation info available

MeSH

Amino AcidsAnimalsBlotting, WesternColeopteraElectrophoresis, Polyacrylamide GelMolecular StructurePlant ProteinsSeed Storage ProteinsSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7581039

Citation

Macedo, M L., et al. "Purification and Properties of Storage Proteins (vicilins) From Cowpea (Vigna Unguiculata) Seeds Which Are Susceptible or Resistant to the Bruchid Beetle Callosobruchus Maculatus." Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, vol. 28, no. 2, 1995, pp. 183-90.
Macedo ML, Fernandes KV, Sales MP, et al. Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus. Braz J Med Biol Res. 1995;28(2):183-90.
Macedo, M. L., Fernandes, K. V., Sales, M. P., & Xavier-Filho, J. (1995). Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus. Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, 28(2), 183-90.
Macedo ML, et al. Purification and Properties of Storage Proteins (vicilins) From Cowpea (Vigna Unguiculata) Seeds Which Are Susceptible or Resistant to the Bruchid Beetle Callosobruchus Maculatus. Braz J Med Biol Res. 1995;28(2):183-90. PubMed PMID: 7581039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus. AU - Macedo,M L, AU - Fernandes,K V, AU - Sales,M P, AU - Xavier-Filho,J, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 183 EP - 90 JF - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JO - Braz J Med Biol Res VL - 28 IS - 2 N2 - Vicilins (7S storage proteins) from seeds of cowpea (Vigna unguiculata) cultivars which are susceptible or resistant to the bruchid beetle C. maculatus were purified by size-exclusion and ion-exchange chromatography. The vicilins were partially characterized by polyacrylamide gel electrophoresis under both denaturing and nondenaturing conditions, by Western blotting and by amino acid analysis. The variant vicilins from C. maculatus-resistant seeds do not differ appreciably from vicilins from susceptible seeds by these criteria except that they are more strongly bound to DEAE-Sepharose, suggesting differences in charge between the various molecules. SN - 0100-879X UR - https://www.unboundmedicine.com/medline/citation/7581039/Purification_and_properties_of_storage_proteins__vicilins__from_cowpea__Vigna_unguiculata__seeds_which_are_susceptible_or_resistant_to_the_bruchid_beetle_Callosobruchus_maculatus_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:7581039 DB - PRIME DP - Unbound Medicine ER -