Tags

Type your tag names separated by a space and hit enter

Polydeoxyribonucleotides and nitric oxide release from guinea-pig hearts during ischaemia and reperfusion.
Br J Pharmacol. 1995 Jun; 115(4):629-35.BJ

Abstract

1. Two polydeoxyribonucleotides, produced by the controlled hydrolysis of DNA of mammalian lung (defibrotide and its lower molecular weight fraction, P.O. 085 DV), were studied for their ability to modify the release of nitrite and the coronary flow in perfusates collected from isolated, normally perfused hearts of guinea-pigs and from hearts subjected to regional ischaemia and reperfusion. 2. In guinea-pig normally perfused hearts, both defibrotide (DFT) and its fraction, P.O. 085 DV, increase the amount of nitrite appearing in perfusates in a concentration-dependent fashion. At the highest concentration studied (10(-6) M), P.O. 085 DV was more effective than DFT. A concomitant increase in the coronary flow was observed. 3. The increase in nitrite in perfusates and the increase in coronary flow induced by both DFT and P.O. 085 DV were significantly reduced by NG-monomethyl-L-arginine (L-NMMA, 10(-4) M), an inhibitor of nitric oxide synthase (NOS). 4. The endothelium-dependent vasodilator, acetylcholine (ACh), enhances the formation of nitrite and the coronary flow. Both the increase in coronary flow and in the formation of nitrite were significantly reduced by L-NMMA (10(-4) M). 5. In guinea-pig hearts subjected to ischaemia and reperfusion, the effect of both compounds in increasing the amount of nitrite in perfusates was more evident and more pronounced with P.O. 085 DV. 6. Reperfusion-induced arrhythmias were significantly reduced by both compounds to the extent of complete protection afforded by compound P.O. 085 DV. 7. The cardioprotective and antiarrhythmic effects of DFT and P.O. 085 DV are discussed.

Authors+Show Affiliations

Department of Preclinical and Clinical Pharmacology, M. Aiazzi-Mancini, Florence University, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7582482

Citation

Masini, E, et al. "Polydeoxyribonucleotides and Nitric Oxide Release From Guinea-pig Hearts During Ischaemia and Reperfusion." British Journal of Pharmacology, vol. 115, no. 4, 1995, pp. 629-35.
Masini E, Lupini M, Mugnai L, et al. Polydeoxyribonucleotides and nitric oxide release from guinea-pig hearts during ischaemia and reperfusion. Br J Pharmacol. 1995;115(4):629-35.
Masini, E., Lupini, M., Mugnai, L., Raspanti, S., & Mannaioni, P. F. (1995). Polydeoxyribonucleotides and nitric oxide release from guinea-pig hearts during ischaemia and reperfusion. British Journal of Pharmacology, 115(4), 629-35.
Masini E, et al. Polydeoxyribonucleotides and Nitric Oxide Release From Guinea-pig Hearts During Ischaemia and Reperfusion. Br J Pharmacol. 1995;115(4):629-35. PubMed PMID: 7582482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polydeoxyribonucleotides and nitric oxide release from guinea-pig hearts during ischaemia and reperfusion. AU - Masini,E, AU - Lupini,M, AU - Mugnai,L, AU - Raspanti,S, AU - Mannaioni,P F, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 629 EP - 35 JF - British journal of pharmacology JO - Br. J. Pharmacol. VL - 115 IS - 4 N2 - 1. Two polydeoxyribonucleotides, produced by the controlled hydrolysis of DNA of mammalian lung (defibrotide and its lower molecular weight fraction, P.O. 085 DV), were studied for their ability to modify the release of nitrite and the coronary flow in perfusates collected from isolated, normally perfused hearts of guinea-pigs and from hearts subjected to regional ischaemia and reperfusion. 2. In guinea-pig normally perfused hearts, both defibrotide (DFT) and its fraction, P.O. 085 DV, increase the amount of nitrite appearing in perfusates in a concentration-dependent fashion. At the highest concentration studied (10(-6) M), P.O. 085 DV was more effective than DFT. A concomitant increase in the coronary flow was observed. 3. The increase in nitrite in perfusates and the increase in coronary flow induced by both DFT and P.O. 085 DV were significantly reduced by NG-monomethyl-L-arginine (L-NMMA, 10(-4) M), an inhibitor of nitric oxide synthase (NOS). 4. The endothelium-dependent vasodilator, acetylcholine (ACh), enhances the formation of nitrite and the coronary flow. Both the increase in coronary flow and in the formation of nitrite were significantly reduced by L-NMMA (10(-4) M). 5. In guinea-pig hearts subjected to ischaemia and reperfusion, the effect of both compounds in increasing the amount of nitrite in perfusates was more evident and more pronounced with P.O. 085 DV. 6. Reperfusion-induced arrhythmias were significantly reduced by both compounds to the extent of complete protection afforded by compound P.O. 085 DV. 7. The cardioprotective and antiarrhythmic effects of DFT and P.O. 085 DV are discussed. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/7582482/Polydeoxyribonucleotides_and_nitric_oxide_release_from_guinea_pig_hearts_during_ischaemia_and_reperfusion_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1995&volume=115&issue=4&spage=629 DB - PRIME DP - Unbound Medicine ER -