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Secretion of the eosinophil-active cytokines interleukin-5, granulocyte/macrophage colony-stimulating factor and interleukin-3 by bronchoalveolar lavage CD4+ and CD8+ T cell lines in atopic asthmatics, and atopic and non-atopic controls.
Eur J Immunol. 1995 Oct; 25(10):2727-31.EJ

Abstract

Specific eosinophil accumulation and activation within the asthmatic bronchial mucosa are thought to occur at least partly through the actions of cytokines, including interleukin (IL)-5, IL-3 and granulocyte/macrophage colony-stimulating factor (GM-CSF). Although mRNA encoding some of these cytokines has been demonstrated in bronchoalveolar lavage (BAL) fluid cells and bronchial biopsies from asthmatics, it has yet to be established whether these cells produce the translated products and whether expression is associated with CD4+ T helper or CD8+ cytotoxic T cells. We addressed this problem by raising polyclonal CD4+ and CD8+ T cell lines from the BAL fluid of six atopic asthmatics, five atopic non-asthmatics and seven non-atopic non-asthmatic controls. BAL fluid cells obtained at fiberoptic bronchoscopy were depleted of adherent cells, and then T lymphocytes expanded by stimulation with monoclonal anti-CD3 antibody and recombinant human IL-2. When lymphocytes had expanded to sufficient numbers, CD4+ and CD8+ cells were separated by positive selection with magnetic beads coated with anti-CD4 or anti-CD8 monoclonal antibodies and further expanded. Cytokine secretion by standardized cell numbers was measured by enzyme-linked immunosorbent assays. BAL CD4+ T cell lines from the asthmatics secreted significantly elevated quantities of both IL-5 and GM-CSF as compared with lines from the atopic and non-atopic controls (p = 0.023-0.003). In contrast, IL-3 secretion did not significantly differ between the groups. In some subjects, CD8+ T cell lines also secreted significant quantities of these cytokines and there was a trend for IL-5 secretion by these cells to be higher in asthmatics than non-atopic controls (p = 0.035). These data are consistent with the hypothesis that activated T lymphocytes from asthmatics, particularly of the CD4+ subset, are predisposed to release elevated quantities of cytokines relevant to the accumulation and activation of eosinophils.

Authors+Show Affiliations

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, GB.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7589063

Citation

Till, S, et al. "Secretion of the Eosinophil-active Cytokines Interleukin-5, Granulocyte/macrophage Colony-stimulating Factor and Interleukin-3 By Bronchoalveolar Lavage CD4+ and CD8+ T Cell Lines in Atopic Asthmatics, and Atopic and Non-atopic Controls." European Journal of Immunology, vol. 25, no. 10, 1995, pp. 2727-31.
Till S, Li B, Durham S, et al. Secretion of the eosinophil-active cytokines interleukin-5, granulocyte/macrophage colony-stimulating factor and interleukin-3 by bronchoalveolar lavage CD4+ and CD8+ T cell lines in atopic asthmatics, and atopic and non-atopic controls. Eur J Immunol. 1995;25(10):2727-31.
Till, S., Li, B., Durham, S., Humbert, M., Assoufi, B., Huston, D., Dickason, R., Jeannin, P., Kay, A. B., & Corrigan, C. (1995). Secretion of the eosinophil-active cytokines interleukin-5, granulocyte/macrophage colony-stimulating factor and interleukin-3 by bronchoalveolar lavage CD4+ and CD8+ T cell lines in atopic asthmatics, and atopic and non-atopic controls. European Journal of Immunology, 25(10), 2727-31.
Till S, et al. Secretion of the Eosinophil-active Cytokines Interleukin-5, Granulocyte/macrophage Colony-stimulating Factor and Interleukin-3 By Bronchoalveolar Lavage CD4+ and CD8+ T Cell Lines in Atopic Asthmatics, and Atopic and Non-atopic Controls. Eur J Immunol. 1995;25(10):2727-31. PubMed PMID: 7589063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secretion of the eosinophil-active cytokines interleukin-5, granulocyte/macrophage colony-stimulating factor and interleukin-3 by bronchoalveolar lavage CD4+ and CD8+ T cell lines in atopic asthmatics, and atopic and non-atopic controls. AU - Till,S, AU - Li,B, AU - Durham,S, AU - Humbert,M, AU - Assoufi,B, AU - Huston,D, AU - Dickason,R, AU - Jeannin,P, AU - Kay,A B, AU - Corrigan,C, PY - 1995/10/1/pubmed PY - 1995/10/1/medline PY - 1995/10/1/entrez SP - 2727 EP - 31 JF - European journal of immunology JO - Eur J Immunol VL - 25 IS - 10 N2 - Specific eosinophil accumulation and activation within the asthmatic bronchial mucosa are thought to occur at least partly through the actions of cytokines, including interleukin (IL)-5, IL-3 and granulocyte/macrophage colony-stimulating factor (GM-CSF). Although mRNA encoding some of these cytokines has been demonstrated in bronchoalveolar lavage (BAL) fluid cells and bronchial biopsies from asthmatics, it has yet to be established whether these cells produce the translated products and whether expression is associated with CD4+ T helper or CD8+ cytotoxic T cells. We addressed this problem by raising polyclonal CD4+ and CD8+ T cell lines from the BAL fluid of six atopic asthmatics, five atopic non-asthmatics and seven non-atopic non-asthmatic controls. BAL fluid cells obtained at fiberoptic bronchoscopy were depleted of adherent cells, and then T lymphocytes expanded by stimulation with monoclonal anti-CD3 antibody and recombinant human IL-2. When lymphocytes had expanded to sufficient numbers, CD4+ and CD8+ cells were separated by positive selection with magnetic beads coated with anti-CD4 or anti-CD8 monoclonal antibodies and further expanded. Cytokine secretion by standardized cell numbers was measured by enzyme-linked immunosorbent assays. BAL CD4+ T cell lines from the asthmatics secreted significantly elevated quantities of both IL-5 and GM-CSF as compared with lines from the atopic and non-atopic controls (p = 0.023-0.003). In contrast, IL-3 secretion did not significantly differ between the groups. In some subjects, CD8+ T cell lines also secreted significant quantities of these cytokines and there was a trend for IL-5 secretion by these cells to be higher in asthmatics than non-atopic controls (p = 0.035). These data are consistent with the hypothesis that activated T lymphocytes from asthmatics, particularly of the CD4+ subset, are predisposed to release elevated quantities of cytokines relevant to the accumulation and activation of eosinophils. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/7589063/Secretion_of_the_eosinophil_active_cytokines_interleukin_5_granulocyte/macrophage_colony_stimulating_factor_and_interleukin_3_by_bronchoalveolar_lavage_CD4+_and_CD8+_T_cell_lines_in_atopic_asthmatics_and_atopic_and_non_atopic_controls_ L2 - https://doi.org/10.1002/eji.1830251002 DB - PRIME DP - Unbound Medicine ER -