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Differential effects of granulocyte-macrophage colony-stimulating factor on eosinophil and neutrophil superoxide anion generation.
J Immunol. 1995 Nov 15; 155(10):4948-54.JI

Abstract

Eosinophils (EOS) are specifically recruited to sites of allergic inflammation and parasitic infection, while neutrophil (NEUT) influx predominates in bacterial infections. This biologic selectivity suggests that granulocytes may respond differently to inflammatory mediators such as granulocyte-macrophage-CSF (GM-CSF). To establish the mechanisms of the response of granulocytes to GM-CSF, isolated human peripheral blood EOS and NEUT were obtained from allergic rhinitis patients and incubated in vitro with this cytokine. Incubation with GM-CSF (10 or 100 pM) significantly enhanced FMLP-stimulated EOS superoxide anion (O2-) generation, LTC4 release, and adhesion to tissue culture plates. Both GM-CSF-enhanced EOS adhesion and O2- generation were inhibited by an anti-beta 2 (CD18) Ab suggesting that this beta 2 integrin was associated with increased cell function. In contrast, FMLP + cytochalasin B were required to demonstrate GM-CSF enhancement of NEUT O2- and LTB4 generation; GM-CSF had no effect on NEUT adhesion. Furthermore, GM-CSF augmentation of FMLP + cytochalasin B-activated NEUT O2- generation was not affected by anti-beta 2 Ab but was blocked by a 5-lipoxygenase-activating protein antagonist, BAY x 1005. Finally, 0.1 to 10 nM LTB4 mimicked the GM-CSF-priming effect on NEUT O2- generation, thus suggesting that the augmented NEUT respiratory burst was the result of LTB4 production and its effect on the NEUT. These data demonstrate that GM-CSF promotes EOS and NEUT O2- generation to FMLP via distinct mechanisms: enhanced adhesion of EOS vs autocrine-priming by enhanced LTB4 generation of NEUT.

Authors+Show Affiliations

Department of Medicine, University of Wisconsin Medical School, Madison 53792, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7594500

Citation

Nagata, M, et al. "Differential Effects of Granulocyte-macrophage Colony-stimulating Factor On Eosinophil and Neutrophil Superoxide Anion Generation." Journal of Immunology (Baltimore, Md. : 1950), vol. 155, no. 10, 1995, pp. 4948-54.
Nagata M, Sedgwick JB, Busse WW. Differential effects of granulocyte-macrophage colony-stimulating factor on eosinophil and neutrophil superoxide anion generation. J Immunol. 1995;155(10):4948-54.
Nagata, M., Sedgwick, J. B., & Busse, W. W. (1995). Differential effects of granulocyte-macrophage colony-stimulating factor on eosinophil and neutrophil superoxide anion generation. Journal of Immunology (Baltimore, Md. : 1950), 155(10), 4948-54.
Nagata M, Sedgwick JB, Busse WW. Differential Effects of Granulocyte-macrophage Colony-stimulating Factor On Eosinophil and Neutrophil Superoxide Anion Generation. J Immunol. 1995 Nov 15;155(10):4948-54. PubMed PMID: 7594500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effects of granulocyte-macrophage colony-stimulating factor on eosinophil and neutrophil superoxide anion generation. AU - Nagata,M, AU - Sedgwick,J B, AU - Busse,W W, PY - 1995/11/15/pubmed PY - 1995/11/15/medline PY - 1995/11/15/entrez SP - 4948 EP - 54 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 155 IS - 10 N2 - Eosinophils (EOS) are specifically recruited to sites of allergic inflammation and parasitic infection, while neutrophil (NEUT) influx predominates in bacterial infections. This biologic selectivity suggests that granulocytes may respond differently to inflammatory mediators such as granulocyte-macrophage-CSF (GM-CSF). To establish the mechanisms of the response of granulocytes to GM-CSF, isolated human peripheral blood EOS and NEUT were obtained from allergic rhinitis patients and incubated in vitro with this cytokine. Incubation with GM-CSF (10 or 100 pM) significantly enhanced FMLP-stimulated EOS superoxide anion (O2-) generation, LTC4 release, and adhesion to tissue culture plates. Both GM-CSF-enhanced EOS adhesion and O2- generation were inhibited by an anti-beta 2 (CD18) Ab suggesting that this beta 2 integrin was associated with increased cell function. In contrast, FMLP + cytochalasin B were required to demonstrate GM-CSF enhancement of NEUT O2- and LTB4 generation; GM-CSF had no effect on NEUT adhesion. Furthermore, GM-CSF augmentation of FMLP + cytochalasin B-activated NEUT O2- generation was not affected by anti-beta 2 Ab but was blocked by a 5-lipoxygenase-activating protein antagonist, BAY x 1005. Finally, 0.1 to 10 nM LTB4 mimicked the GM-CSF-priming effect on NEUT O2- generation, thus suggesting that the augmented NEUT respiratory burst was the result of LTB4 production and its effect on the NEUT. These data demonstrate that GM-CSF promotes EOS and NEUT O2- generation to FMLP via distinct mechanisms: enhanced adhesion of EOS vs autocrine-priming by enhanced LTB4 generation of NEUT. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7594500/Differential_effects_of_granulocyte_macrophage_colony_stimulating_factor_on_eosinophil_and_neutrophil_superoxide_anion_generation_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7594500 DB - PRIME DP - Unbound Medicine ER -