Tags

Type your tag names separated by a space and hit enter

Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe.
Proc Natl Acad Sci U S A. 1995 Jun 20; 92(13):6180-4.PN

Abstract

We describe a protein kinase, Shk1, from the fission yeast Schizosaccharomyces pombe, which is structurally related to the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases. We provide genetic evidence for physical and functional interaction between Shk1 and the Cdc42 GTP-binding protein required for normal cell morphology and mating in S. pombe. We further show that expression of the STE20 gene complements the shk1 null mutation and that Shk1 is capable of signaling to the pheromone-responsive mitogen-activated protein kinase cascade in S. cerevisiae. Our results lead us to propose that signaling modules composed of small GTP-binding proteins and protein kinases related to Shk1, Ste20, and p65PAK, are highly conserved in evolution and participate in both cytoskeletal functions and mitogen-activated protein kinase signaling pathways.

Authors+Show Affiliations

Cold Spring Harbor Laboratory, NY 11724, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7597098

Citation

Marcus, S, et al. "Shk1, a Homolog of the Saccharomyces Cerevisiae Ste20 and Mammalian p65PAK Protein Kinases, Is a Component of a Ras/Cdc42 Signaling Module in the Fission Yeast Schizosaccharomyces Pombe." Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 13, 1995, pp. 6180-4.
Marcus S, Polverino A, Chang E, et al. Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc Natl Acad Sci U S A. 1995;92(13):6180-4.
Marcus, S., Polverino, A., Chang, E., Robbins, D., Cobb, M. H., & Wigler, M. H. (1995). Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences of the United States of America, 92(13), 6180-4.
Marcus S, et al. Shk1, a Homolog of the Saccharomyces Cerevisiae Ste20 and Mammalian p65PAK Protein Kinases, Is a Component of a Ras/Cdc42 Signaling Module in the Fission Yeast Schizosaccharomyces Pombe. Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6180-4. PubMed PMID: 7597098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. AU - Marcus,S, AU - Polverino,A, AU - Chang,E, AU - Robbins,D, AU - Cobb,M H, AU - Wigler,M H, PY - 1995/6/20/pubmed PY - 1995/6/20/medline PY - 1995/6/20/entrez SP - 6180 EP - 4 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 92 IS - 13 N2 - We describe a protein kinase, Shk1, from the fission yeast Schizosaccharomyces pombe, which is structurally related to the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases. We provide genetic evidence for physical and functional interaction between Shk1 and the Cdc42 GTP-binding protein required for normal cell morphology and mating in S. pombe. We further show that expression of the STE20 gene complements the shk1 null mutation and that Shk1 is capable of signaling to the pheromone-responsive mitogen-activated protein kinase cascade in S. cerevisiae. Our results lead us to propose that signaling modules composed of small GTP-binding proteins and protein kinases related to Shk1, Ste20, and p65PAK, are highly conserved in evolution and participate in both cytoskeletal functions and mitogen-activated protein kinase signaling pathways. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/7597098/Shk1_a_homolog_of_the_Saccharomyces_cerevisiae_Ste20_and_mammalian_p65PAK_protein_kinases_is_a_component_of_a_Ras/Cdc42_signaling_module_in_the_fission_yeast_Schizosaccharomyces_pombe_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=7597098 DB - PRIME DP - Unbound Medicine ER -