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Alterations in murine fetal thymus and liver hematopoietic cell populations following developmental exposure to 7,12-dimethylbenz[a]anthracene.
Environ Res. 1995 Feb; 68(2):106-13.ER

Abstract

Gestational exposure of ICR mice to the environmental contaminant 7,12-dimethylbenz[a]anthracene (DMBA) was used (i) to study the developmental immunotoxicity of this chemical agent and (ii) to evaluate potential hematopoietic cellular targets in a sensitive developmental model which may be involved in immunosuppression induced by this carcinogenic polycyclic aromatic hydrocarbon (PAH). DMBA produced a dose-dependent hypocellularity in both fetal mouse thymus and liver. Resident hematopoietic cell subpopulations in fetal liver, identified by CD44, CD45R, and Mac-1 monoclonal antibody binding, were reduced by the gestational DMBA treatment. In particular, the total number of CD45R+ B-lineage lymphocytic cells in fetal liver was reduced to 20% of control levels by DMBA. Unlike previous reports with related PAH, DMBA did not inhibit thymocyte differentiation, as indicated by unaltered thymocyte expression of CD4, CD8, and heat-stable antigens. These data may indicate that production of thymic atrophy and impairment of thymocyte differentiation by PAH involve separate mechanisms of action. Results of the present study additionally identify changes in immune cell populations that correlate well with inhibition of cell- and humoral-mediated immunity in experimental animals treated with DMBA.

Authors+Show Affiliations

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University 24061-0442, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7601071

Citation

Holladay, S D., and B J. Smith. "Alterations in Murine Fetal Thymus and Liver Hematopoietic Cell Populations Following Developmental Exposure to 7,12-dimethylbenz[a]anthracene." Environmental Research, vol. 68, no. 2, 1995, pp. 106-13.
Holladay SD, Smith BJ. Alterations in murine fetal thymus and liver hematopoietic cell populations following developmental exposure to 7,12-dimethylbenz[a]anthracene. Environ Res. 1995;68(2):106-13.
Holladay, S. D., & Smith, B. J. (1995). Alterations in murine fetal thymus and liver hematopoietic cell populations following developmental exposure to 7,12-dimethylbenz[a]anthracene. Environmental Research, 68(2), 106-13.
Holladay SD, Smith BJ. Alterations in Murine Fetal Thymus and Liver Hematopoietic Cell Populations Following Developmental Exposure to 7,12-dimethylbenz[a]anthracene. Environ Res. 1995;68(2):106-13. PubMed PMID: 7601071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations in murine fetal thymus and liver hematopoietic cell populations following developmental exposure to 7,12-dimethylbenz[a]anthracene. AU - Holladay,S D, AU - Smith,B J, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 106 EP - 13 JF - Environmental research JO - Environ Res VL - 68 IS - 2 N2 - Gestational exposure of ICR mice to the environmental contaminant 7,12-dimethylbenz[a]anthracene (DMBA) was used (i) to study the developmental immunotoxicity of this chemical agent and (ii) to evaluate potential hematopoietic cellular targets in a sensitive developmental model which may be involved in immunosuppression induced by this carcinogenic polycyclic aromatic hydrocarbon (PAH). DMBA produced a dose-dependent hypocellularity in both fetal mouse thymus and liver. Resident hematopoietic cell subpopulations in fetal liver, identified by CD44, CD45R, and Mac-1 monoclonal antibody binding, were reduced by the gestational DMBA treatment. In particular, the total number of CD45R+ B-lineage lymphocytic cells in fetal liver was reduced to 20% of control levels by DMBA. Unlike previous reports with related PAH, DMBA did not inhibit thymocyte differentiation, as indicated by unaltered thymocyte expression of CD4, CD8, and heat-stable antigens. These data may indicate that production of thymic atrophy and impairment of thymocyte differentiation by PAH involve separate mechanisms of action. Results of the present study additionally identify changes in immune cell populations that correlate well with inhibition of cell- and humoral-mediated immunity in experimental animals treated with DMBA. SN - 0013-9351 UR - https://www.unboundmedicine.com/medline/citation/7601071/Alterations_in_murine_fetal_thymus_and_liver_hematopoietic_cell_populations_following_developmental_exposure_to_712_dimethylbenz[a]anthracene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0013-9351(85)71014-6 DB - PRIME DP - Unbound Medicine ER -