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Quail myoD is regulated by a complex array of cis-acting control sequences.
Dev Biol. 1995 Jul; 170(1):21-38.DB

Abstract

Quail myoD (QmyoD) is the earliest myoD family member expressed in quail somites and its transcription is initiated in response to early developmental signals. We have investigated the transcriptional regulation of QmyoD to define the cis-acting sequences required for tissue-specific and correct developmental expression. The QmyoD gene locus was isolated and sequenced and its regulatory properties were characterized. We identified three distinct regions of cis-acting regulatory sequences that control the expression of reporter gene constructs following DNA transfection into cell lines and cultured primary quail cells. The first, a complex distal control region (DCR), 11.5 kb upstream of the gene, contains three separable enhancer activities. Two of these DCR enhancer activities are tissue specific and can be autoactivated. In addition, these same two enhancers and the entire DCR direct somite- and muscle-specific expression of a reporter gene in transgenic mice. Sequence analysis of the DCR enhancers reveals clusters of E-boxes, MEF2 binding motifs, and the stretches of sequence identity with the human myoD enhancer. Second, the promoter region has sequences which act positively to direct expression in both muscle and nonmuscle cells as well as sequences that repress expression specifically in nonmuscle cells. The third control region, the PR, is located -3.3 to -5 kb from the transcription start site and directs muscle-specific expression in cultured cells. This analysis demonstrates that QmyoD has multiple control regions and that some features of myoD regulation are conserved between mammals and birds.

Authors+Show Affiliations

Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7601311

Citation

Pinney, D F., et al. "Quail myoD Is Regulated By a Complex Array of Cis-acting Control Sequences." Developmental Biology, vol. 170, no. 1, 1995, pp. 21-38.
Pinney DF, de la Brousse FC, Faerman A, et al. Quail myoD is regulated by a complex array of cis-acting control sequences. Dev Biol. 1995;170(1):21-38.
Pinney, D. F., de la Brousse, F. C., Faerman, A., Shani, M., Maruyama, K., & Emerson, C. P. (1995). Quail myoD is regulated by a complex array of cis-acting control sequences. Developmental Biology, 170(1), 21-38.
Pinney DF, et al. Quail myoD Is Regulated By a Complex Array of Cis-acting Control Sequences. Dev Biol. 1995;170(1):21-38. PubMed PMID: 7601311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quail myoD is regulated by a complex array of cis-acting control sequences. AU - Pinney,D F, AU - de la Brousse,F C, AU - Faerman,A, AU - Shani,M, AU - Maruyama,K, AU - Emerson,C P,Jr PY - 1995/7/1/pubmed PY - 2001/3/28/medline PY - 1995/7/1/entrez SP - 21 EP - 38 JF - Developmental biology JO - Dev Biol VL - 170 IS - 1 N2 - Quail myoD (QmyoD) is the earliest myoD family member expressed in quail somites and its transcription is initiated in response to early developmental signals. We have investigated the transcriptional regulation of QmyoD to define the cis-acting sequences required for tissue-specific and correct developmental expression. The QmyoD gene locus was isolated and sequenced and its regulatory properties were characterized. We identified three distinct regions of cis-acting regulatory sequences that control the expression of reporter gene constructs following DNA transfection into cell lines and cultured primary quail cells. The first, a complex distal control region (DCR), 11.5 kb upstream of the gene, contains three separable enhancer activities. Two of these DCR enhancer activities are tissue specific and can be autoactivated. In addition, these same two enhancers and the entire DCR direct somite- and muscle-specific expression of a reporter gene in transgenic mice. Sequence analysis of the DCR enhancers reveals clusters of E-boxes, MEF2 binding motifs, and the stretches of sequence identity with the human myoD enhancer. Second, the promoter region has sequences which act positively to direct expression in both muscle and nonmuscle cells as well as sequences that repress expression specifically in nonmuscle cells. The third control region, the PR, is located -3.3 to -5 kb from the transcription start site and directs muscle-specific expression in cultured cells. This analysis demonstrates that QmyoD has multiple control regions and that some features of myoD regulation are conserved between mammals and birds. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/7601311/Quail_myoD_is_regulated_by_a_complex_array_of_cis_acting_control_sequences_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(85)71192-X DB - PRIME DP - Unbound Medicine ER -