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TGF-beta induces expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 via transcriptional factor AP-1 induced by protein kinase in osteoblastic cells.
J Immunol. 1995 Jul 01; 155(1):419-26.JI

Abstract

In this work, we demonstrate the signal-transducing mechanism of TGF-beta 1 for gene expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 (MCP-1) in clonal osteoblastic MC3T3-E1 cells. TGF-beta 1-induced JE/MCP-1 gene expression in the cells was inhibited markedly by H-7 (1-(5-isoguinolinesulfonyl)-2-O-methylpiperazine-dihydrochloride) and staurosporine, potent inhibitors of protein kinase. TGF-beta 1-induced expression of both early proto-oncogenes c-fos and c-jun in the cells was also inhibited by H-7 and staurosporine. Antisense oligonucleotides to c-fos and c-jun genes inhibited significantly the cytokine-induced JE/MCP-1 gene expression. Curcumin, a specific inhibitor of c-jun/AP-1, inhibited the cytokine-induced c-jun gene expression in a dose-dependent manner, though the c-fos gene expression was not affected. TGF-beta 1 stimulated transcriptionally the JE/MCP-1 gene expression, and this stimulation was inhibited significantly by curcumin. Curcumin-induced inhibition of the JE/MCP-1 gene product was also evidenced by both an assay involving immunoprecipitation with antiserum specific for JE/MCP-1 and an assay for monocyte chemotaxis. Curcumin markedly inhibited AP-1 binding activity to 12-tetradecanoyl phorbol-13-acetate-responsive element (TRE) in the cytokine-treated cells. Furthermore, H-7 and staurosporine also inhibited the binding activity to TRE in the cells treated by the cytokine. These results demonstrate that TGF-beta 1 induces expression of monocyte chemoattractant JE/MCP-1 via the transcriptional factor AP-1 induced by protein kinase in the osteoblastic cells.

Authors+Show Affiliations

Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7602115

Citation

Takeshita, A, et al. "TGF-beta Induces Expression of Monocyte Chemoattractant JE/monocyte Chemoattractant Protein 1 Via Transcriptional Factor AP-1 Induced By Protein Kinase in Osteoblastic Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 155, no. 1, 1995, pp. 419-26.
Takeshita A, Chen Y, Watanabe A, et al. TGF-beta induces expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 via transcriptional factor AP-1 induced by protein kinase in osteoblastic cells. J Immunol. 1995;155(1):419-26.
Takeshita, A., Chen, Y., Watanabe, A., Kitano, S., & Hanazawa, S. (1995). TGF-beta induces expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 via transcriptional factor AP-1 induced by protein kinase in osteoblastic cells. Journal of Immunology (Baltimore, Md. : 1950), 155(1), 419-26.
Takeshita A, et al. TGF-beta Induces Expression of Monocyte Chemoattractant JE/monocyte Chemoattractant Protein 1 Via Transcriptional Factor AP-1 Induced By Protein Kinase in Osteoblastic Cells. J Immunol. 1995 Jul 1;155(1):419-26. PubMed PMID: 7602115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TGF-beta induces expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 via transcriptional factor AP-1 induced by protein kinase in osteoblastic cells. AU - Takeshita,A, AU - Chen,Y, AU - Watanabe,A, AU - Kitano,S, AU - Hanazawa,S, PY - 1995/7/1/pubmed PY - 1995/7/1/medline PY - 1995/7/1/entrez SP - 419 EP - 26 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 155 IS - 1 N2 - In this work, we demonstrate the signal-transducing mechanism of TGF-beta 1 for gene expression of monocyte chemoattractant JE/monocyte chemoattractant protein 1 (MCP-1) in clonal osteoblastic MC3T3-E1 cells. TGF-beta 1-induced JE/MCP-1 gene expression in the cells was inhibited markedly by H-7 (1-(5-isoguinolinesulfonyl)-2-O-methylpiperazine-dihydrochloride) and staurosporine, potent inhibitors of protein kinase. TGF-beta 1-induced expression of both early proto-oncogenes c-fos and c-jun in the cells was also inhibited by H-7 and staurosporine. Antisense oligonucleotides to c-fos and c-jun genes inhibited significantly the cytokine-induced JE/MCP-1 gene expression. Curcumin, a specific inhibitor of c-jun/AP-1, inhibited the cytokine-induced c-jun gene expression in a dose-dependent manner, though the c-fos gene expression was not affected. TGF-beta 1 stimulated transcriptionally the JE/MCP-1 gene expression, and this stimulation was inhibited significantly by curcumin. Curcumin-induced inhibition of the JE/MCP-1 gene product was also evidenced by both an assay involving immunoprecipitation with antiserum specific for JE/MCP-1 and an assay for monocyte chemotaxis. Curcumin markedly inhibited AP-1 binding activity to 12-tetradecanoyl phorbol-13-acetate-responsive element (TRE) in the cytokine-treated cells. Furthermore, H-7 and staurosporine also inhibited the binding activity to TRE in the cells treated by the cytokine. These results demonstrate that TGF-beta 1 induces expression of monocyte chemoattractant JE/MCP-1 via the transcriptional factor AP-1 induced by protein kinase in the osteoblastic cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7602115/TGF_beta_induces_expression_of_monocyte_chemoattractant_JE/monocyte_chemoattractant_protein_1_via_transcriptional_factor_AP_1_induced_by_protein_kinase_in_osteoblastic_cells_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7602115 DB - PRIME DP - Unbound Medicine ER -