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Evolutionarily conserved structural elements are critical for processing of Internal Transcribed Spacer 2 from Saccharomyces cerevisiae precursor ribosomal RNA.
J Mol Biol. 1995 Jun 30; 250(1):24-36.JM

Abstract

Structural features of Internal Transcribed Spacer 2 (ITS2) important for the correct and efficient removal of this spacer from Saccharomyces cerevisiae pre-rRNA were identified by in vivo mutational analysis based upon phylogenetic comparison with its counterparts from four different yeast species. Compatibility between ITS2 structure and the S. cerevisiae processing machinery was found to have been maintained over only a short evolutionary distance, in contrast to the situation for ITS1. Nevertheless, cis-acting elements required for correct and efficient processing are confined predominantly to those regions of the spacer that show the highest degree of evolutionary conservation. Mutation or deletion of each of these regions severely reduced production of mature 26 S, but not 17 S rRNA, mainly by impeding processing of the 29 SB precursor. In some cases, however, conversion of 29SA into 29 SB pre-rRNA also appeared to be affected. Deletion of non-conserved segments, on the other hand, caused little or no disturbance in processing. Surprisingly, some combinations of such individually neutral deletions had a severe negative effect on the removal of ITS2, suggesting a requirement for a higher-order structure of ITS2. Finally, even structural alterations of ITS2 that did not noticeably affect processing, significantly reduced the growth rate of cells that exclusively express the mutant rDNA units. We take this as further evidence for a direct role of ITS2 in the formation of fully functional 60 S ribosomal subunits.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology IMBW, BioCentrum Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7602595

Citation

van Nues, R W., et al. "Evolutionarily Conserved Structural Elements Are Critical for Processing of Internal Transcribed Spacer 2 From Saccharomyces Cerevisiae Precursor Ribosomal RNA." Journal of Molecular Biology, vol. 250, no. 1, 1995, pp. 24-36.
van Nues RW, Rientjes JM, Morré SA, et al. Evolutionarily conserved structural elements are critical for processing of Internal Transcribed Spacer 2 from Saccharomyces cerevisiae precursor ribosomal RNA. J Mol Biol. 1995;250(1):24-36.
van Nues, R. W., Rientjes, J. M., Morré, S. A., Mollee, E., Planta, R. J., Venema, J., & Raué, H. A. (1995). Evolutionarily conserved structural elements are critical for processing of Internal Transcribed Spacer 2 from Saccharomyces cerevisiae precursor ribosomal RNA. Journal of Molecular Biology, 250(1), 24-36.
van Nues RW, et al. Evolutionarily Conserved Structural Elements Are Critical for Processing of Internal Transcribed Spacer 2 From Saccharomyces Cerevisiae Precursor Ribosomal RNA. J Mol Biol. 1995 Jun 30;250(1):24-36. PubMed PMID: 7602595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evolutionarily conserved structural elements are critical for processing of Internal Transcribed Spacer 2 from Saccharomyces cerevisiae precursor ribosomal RNA. AU - van Nues,R W, AU - Rientjes,J M, AU - Morré,S A, AU - Mollee,E, AU - Planta,R J, AU - Venema,J, AU - Raué,H A, PY - 1995/6/30/pubmed PY - 1995/6/30/medline PY - 1995/6/30/entrez SP - 24 EP - 36 JF - Journal of molecular biology JO - J Mol Biol VL - 250 IS - 1 N2 - Structural features of Internal Transcribed Spacer 2 (ITS2) important for the correct and efficient removal of this spacer from Saccharomyces cerevisiae pre-rRNA were identified by in vivo mutational analysis based upon phylogenetic comparison with its counterparts from four different yeast species. Compatibility between ITS2 structure and the S. cerevisiae processing machinery was found to have been maintained over only a short evolutionary distance, in contrast to the situation for ITS1. Nevertheless, cis-acting elements required for correct and efficient processing are confined predominantly to those regions of the spacer that show the highest degree of evolutionary conservation. Mutation or deletion of each of these regions severely reduced production of mature 26 S, but not 17 S rRNA, mainly by impeding processing of the 29 SB precursor. In some cases, however, conversion of 29SA into 29 SB pre-rRNA also appeared to be affected. Deletion of non-conserved segments, on the other hand, caused little or no disturbance in processing. Surprisingly, some combinations of such individually neutral deletions had a severe negative effect on the removal of ITS2, suggesting a requirement for a higher-order structure of ITS2. Finally, even structural alterations of ITS2 that did not noticeably affect processing, significantly reduced the growth rate of cells that exclusively express the mutant rDNA units. We take this as further evidence for a direct role of ITS2 in the formation of fully functional 60 S ribosomal subunits. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/7602595/Evolutionarily_conserved_structural_elements_are_critical_for_processing_of_Internal_Transcribed_Spacer_2_from_Saccharomyces_cerevisiae_precursor_ribosomal_RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(85)70355-5 DB - PRIME DP - Unbound Medicine ER -