Type your tag names separated by a space and hit enter
Induction of a cytogenetic adaptive response in germ cells of irradiated mice with very low-dose rate of chronic gamma-irradiation and its biological influence on radiation-induced DNA or chromosomal damage and cell killing in their male offspring.
In earlier studies we have shown that either a single exposure or multiple exposures to a low dose of X-rays (0.05 Gy) induced a significant cytogenetic adaptive response in mouse germ cells. In this paper, a very low-dose rate (20 microGy/min) of chronic 60Co gamma-irradiation was used to pre-irradiate mice for 40 days. Then, another 40 days later, these mice were treated with a subsequent large dose of X-irradiation, followed 24 h later by cytogenetic analysis of their spermatocytes. Analysis for radiation-induced DNA and chromosomal damage was also carried out in splenocytes, bone marrow cells and spermatocytes of the offspring of mice adapted by the low-dose rate of chronic gamma-irradiation. Results demonstrated that (i) cumulative gamma-irradiation (1.10 Gy) at the dose rate 20 microGy/min induced a marked cytogenetic adaptive response in the mouse germ cells (stem spermatogonia); (ii) the sensitivity of offspring's bone marrow cells and spermatocytes to 1.5 Gy X-ray-induced chromosome aberrations was not influenced by the low-dose radiation delivered to paternal germ cells; (iii) either constitutive or post-irradiation DNA repair capacity (UV-induced unscheduled DNA synthesis, UDS) was not modified in the offspring's splenocytes; (iv) the sensitivity of the offspring's splenocytes to radiation-induced cell killing was also not altered. These results suggest that low-dose radiation delivered to the male parents with a significant induction of cytogenetic adaptive response in their germ cell does not likely cause any risk of damaging effects to the offspring of those irradiated male mice.
Bone Marrow Cells
Mice, Inbred Strains
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't