Tags

Type your tag names separated by a space and hit enter

Role of protein kinase C isozymes in Fc gamma receptor-mediated intracellular killing of Staphylococcus aureus by human monocytes.
J Immunol. 1995 Jul 15; 155(2):776-84.JI

Abstract

Intracellular killing of Staphylococcus aureus by human monocytes after cross-linking Fc gamma R is known to be a phospholipase C (PLC)-dependent process. Activation of PLC leads to the formation of second messengers that synergistically activate protein kinase C (PKC). The aim of this study was to obtain more insight into the role of PKC in Fc gamma R-mediated killing process. PKC inhibitors H-7 and staurosporine markedly suppressed the killing of S. aureus by monocytes stimulated by cross-linking Fc gamma RI or -II. Cross-linking Fc gamma R caused a transient increase in PKC activity in the membranes of monocytes, as measured by Ca2+/phospholipid-dependent phosphorylation of histone. Western blot analysis revealed that cross-linking Fc gamma R stimulated a transient increase in PKC-beta in the membranes of monocytes with kinetics that correlated closely with the translocation of PKC activity. Cross-linking Fc gamma R on monocytes also stimulated the translocation of PKC-epsilon but not PKC-alpha. PMA and 1-oleoyl-2-acetylglycerol (OAG), which caused translocation of PKC-alpha, -beta, and -epsilon, did not stimulate the killing process. Incubation with these PKC activators for 10 min rendered monocytes unresponsive to stimulation of killing of S. aureus via Fc gamma R. It could be that activation of certain PKC isozymes, probably PKC-alpha and -epsilon, by these activators causes feedback inhibition of PLC and, consequently, the killing in monocytes, because PMA blocks the Fc gamma R-mediated intracellular inositol(1,4,5)P3 formation and PKC translocation. Together, our results indicate that PKC isozymes play an important role in both stimulation and inhibition of the Fc gamma R-mediated intracellular killing of bacteria by monocytes.

Authors+Show Affiliations

Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7608554

Citation

Zheng, L, et al. "Role of Protein Kinase C Isozymes in Fc Gamma Receptor-mediated Intracellular Killing of Staphylococcus Aureus By Human Monocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 155, no. 2, 1995, pp. 776-84.
Zheng L, Zomerdijk TP, Aarnoudse C, et al. Role of protein kinase C isozymes in Fc gamma receptor-mediated intracellular killing of Staphylococcus aureus by human monocytes. J Immunol. 1995;155(2):776-84.
Zheng, L., Zomerdijk, T. P., Aarnoudse, C., van Furth, R., & Nibbering, P. H. (1995). Role of protein kinase C isozymes in Fc gamma receptor-mediated intracellular killing of Staphylococcus aureus by human monocytes. Journal of Immunology (Baltimore, Md. : 1950), 155(2), 776-84.
Zheng L, et al. Role of Protein Kinase C Isozymes in Fc Gamma Receptor-mediated Intracellular Killing of Staphylococcus Aureus By Human Monocytes. J Immunol. 1995 Jul 15;155(2):776-84. PubMed PMID: 7608554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of protein kinase C isozymes in Fc gamma receptor-mediated intracellular killing of Staphylococcus aureus by human monocytes. AU - Zheng,L, AU - Zomerdijk,T P, AU - Aarnoudse,C, AU - van Furth,R, AU - Nibbering,P H, PY - 1995/7/15/pubmed PY - 1995/7/15/medline PY - 1995/7/15/entrez SP - 776 EP - 84 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 155 IS - 2 N2 - Intracellular killing of Staphylococcus aureus by human monocytes after cross-linking Fc gamma R is known to be a phospholipase C (PLC)-dependent process. Activation of PLC leads to the formation of second messengers that synergistically activate protein kinase C (PKC). The aim of this study was to obtain more insight into the role of PKC in Fc gamma R-mediated killing process. PKC inhibitors H-7 and staurosporine markedly suppressed the killing of S. aureus by monocytes stimulated by cross-linking Fc gamma RI or -II. Cross-linking Fc gamma R caused a transient increase in PKC activity in the membranes of monocytes, as measured by Ca2+/phospholipid-dependent phosphorylation of histone. Western blot analysis revealed that cross-linking Fc gamma R stimulated a transient increase in PKC-beta in the membranes of monocytes with kinetics that correlated closely with the translocation of PKC activity. Cross-linking Fc gamma R on monocytes also stimulated the translocation of PKC-epsilon but not PKC-alpha. PMA and 1-oleoyl-2-acetylglycerol (OAG), which caused translocation of PKC-alpha, -beta, and -epsilon, did not stimulate the killing process. Incubation with these PKC activators for 10 min rendered monocytes unresponsive to stimulation of killing of S. aureus via Fc gamma R. It could be that activation of certain PKC isozymes, probably PKC-alpha and -epsilon, by these activators causes feedback inhibition of PLC and, consequently, the killing in monocytes, because PMA blocks the Fc gamma R-mediated intracellular inositol(1,4,5)P3 formation and PKC translocation. Together, our results indicate that PKC isozymes play an important role in both stimulation and inhibition of the Fc gamma R-mediated intracellular killing of bacteria by monocytes. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7608554/Role_of_protein_kinase_C_isozymes_in_Fc_gamma_receptor_mediated_intracellular_killing_of_Staphylococcus_aureus_by_human_monocytes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7608554 DB - PRIME DP - Unbound Medicine ER -