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Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases.
J Struct Biol. 1995 Mar-Apr; 114(2):140-52.JS

Abstract

The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-(1-->3)-glucanase, endo-beta-(-->6)-glucanase, and endo-alpha-(1-->3)-glucanase produced by Bacillus circulans; exo-beta-(1-->3)-glucanase and endo-beta-(1-->3)-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-(1-->3)-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-(1-->3)- and endo-beta-(1-->6)-glucanases of B. circulans to reveal a wall component consisting of densely interwoven microfibrils. The fibrils were hydrolyzed by treatment with the Schiz. japonicus endo-beta-(1-->3)-glucanase followed by B. circulans endo-alpha-(1-->3)-glucanase--suggesting that they were composed of -beta-(1-->3)-linked glucan and alpha-(1-->3)-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining.

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Authors+Show Affiliations

Kopecká M
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Fleet GH
No affiliation info available
Phaff HJ
No affiliation info available

MeSH

Actin CytoskeletonCell WallGlucan 1,3-beta-GlucosidaseGlucansGlycoside HydrolasesMicroscopy, ElectronSchizosaccharomycesbeta-Glucosidase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7612397

Citation

Kopecká, M, et al. "Ultrastructure of the Cell Wall of Schizosaccharomyces Pombe Following Treatment With Various Glucanases." Journal of Structural Biology, vol. 114, no. 2, 1995, pp. 140-52.
Kopecká M, Fleet GH, Phaff HJ. Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases. J Struct Biol. 1995;114(2):140-52.
Kopecká, M., Fleet, G. H., & Phaff, H. J. (1995). Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases. Journal of Structural Biology, 114(2), 140-52.
Kopecká M, Fleet GH, Phaff HJ. Ultrastructure of the Cell Wall of Schizosaccharomyces Pombe Following Treatment With Various Glucanases. J Struct Biol. 1995 Mar-Apr;114(2):140-52. PubMed PMID: 7612397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases. AU - Kopecká,M, AU - Fleet,G H, AU - Phaff,H J, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 140 EP - 52 JF - Journal of structural biology JO - J Struct Biol VL - 114 IS - 2 N2 - The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-(1-->3)-glucanase, endo-beta-(-->6)-glucanase, and endo-alpha-(1-->3)-glucanase produced by Bacillus circulans; exo-beta-(1-->3)-glucanase and endo-beta-(1-->3)-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-(1-->3)-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-(1-->3)- and endo-beta-(1-->6)-glucanases of B. circulans to reveal a wall component consisting of densely interwoven microfibrils. The fibrils were hydrolyzed by treatment with the Schiz. japonicus endo-beta-(1-->3)-glucanase followed by B. circulans endo-alpha-(1-->3)-glucanase--suggesting that they were composed of -beta-(1-->3)-linked glucan and alpha-(1-->3)-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining. SN - 1047-8477 UR - https://www.unboundmedicine.com/medline/citation/7612397/Ultrastructure_of_the_cell_wall_of_Schizosaccharomyces_pombe_following_treatment_with_various_glucanases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1047-8477(85)71013-1 DB - PRIME DP - Unbound Medicine ER -